caa a22 4500 605479275 CHVBK 20210128100409.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s10544-014-9914-9 doi (NATIONALLICENCE)springer-10.1007/s10544-014-9914-9 In vivo imaging of microfluidic-produced microbubbles [Elektronische Daten] [Ali Dhanaliwala, Adam Dixon, Dan Lin, Johnny Chen, Alexander Klibanov, John Hossack] Microfluidics-based production of stable microbubbles for ultrasound contrast enhancement or drug/gene delivery allows for precise control over microbubble diameter but at the cost of a low production rate. In situ microfluidic production of microbubbles directly in the vasculature may eliminate the necessity for high microbubble production rates, long stability, or small diameters. Towards this goal, we investigated whether microfluidic-produced microbubbles directly administered into a mouse tail vein could provide sufficient ultrasound contrast. Microbubbles composed of nitrogen gas and stabilized with 3 % bovine serum albumin and 10 % dextrose were injected for 10 seconds into wild type C57BL/6 mice, via a tail-vein catheter. Short-axis images of the right and left ventricle were acquired at 12.5 MHz and image intensity over time was analyzed. Microbubbles were produced on the order of 105 microbubbles/s and were observed in both the right and left ventricles. The median rise time, duration, and decay time within the right ventricle were 2.9, 21.3, and 14.3 s, respectively. All mice survived the procedure with no observable respiratory or heart rate distress despite microbubble diameters as large as 19 μm. Springer Science+Business Media New York, 2015 Flow-focusing microfluidic device nationallicence Microbubbles nationallicence Ultrasound contrast agents nationallicence Dhanaliwala Ali Department of Biomedical Engineering, University of Virginia, Charlottesville, VA, USA aut Dixon Adam Department of Biomedical Engineering, University of Virginia, Charlottesville, VA, USA aut Lin Dan Department of Biomedical Engineering, University of Virginia, Charlottesville, VA, USA aut Chen Johnny Department of Biomedical Engineering, University of Virginia, Charlottesville, VA, USA aut Klibanov Alexander Department of Medicine - Cardiovascular Division, University of Virginia, Charlottesville, VA, USA aut Hossack John Department of Biomedical Engineering, University of Virginia, Charlottesville, VA, USA aut Biomedical Microdevices Springer US; http://www.springer-ny.com 17/1(2015-02-01), 1-12 1387-2176 17:1<1 2015 17 10544 https://doi.org/10.1007/s10544-014-9914-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10544-014-9914-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dhanaliwala Ali Department of Biomedical Engineering, University of Virginia, Charlottesville, VA, USA aut NATIONALLICENCE 700 1- Dixon Adam Department of Biomedical Engineering, University of Virginia, Charlottesville, VA, USA aut NATIONALLICENCE 700 1- Lin Dan Department of Biomedical Engineering, University of Virginia, Charlottesville, VA, USA aut NATIONALLICENCE 700 1- Chen Johnny Department of Biomedical Engineering, University of Virginia, Charlottesville, VA, USA aut NATIONALLICENCE 700 1- Klibanov Alexander Department of Medicine - Cardiovascular Division, University of Virginia, Charlottesville, VA, USA aut NATIONALLICENCE 700 1- Hossack John Department of Biomedical Engineering, University of Virginia, Charlottesville, VA, USA aut NATIONALLICENCE 773 0- Biomedical Microdevices Springer US; http://www.springer-ny.com 17/1(2015-02-01), 1-12 1387-2176 17:1<1 2015 17 10544