caa a22 4500 605479313 CHVBK 20210128100409.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s10544-014-9915-8 doi (NATIONALLICENCE)springer-10.1007/s10544-014-9915-8 Printing cell-laden gelatin constructs by free-form fabrication and enzymatic protein crosslinking [Elektronische Daten] [Scott Irvine, Animesh Agrawal, Bae Lee, Hui Chua, Kok Low, Boon Lau, Marcelle Machluf, Subbu Venkatraman] Considerable interest has arisen in precision fabrication of cell bearing scaffolds and structures by free form fabrication. Gelatin is an ideal material for creating cell entrapping constructs, yet its application in free form fabrication remains challenging. We demonstrate the use of gelatin, crosslinked with microbial transglutaminase (mTgase), as a material to print cell bearing hydrogels for both 2-dimensional (2-D) precision patterns and 3-dimensional (3-D) constructs. The precision patterning was attained with 3% gelatin and 2% high molecular weight poly (ethylene oxide) (PEO) whereas 3-D constructs were obtained using a 5% gelatin solution. These hydrogels, referred to as "bioinks” supported entrapped cell growth, allowing cell spreading and proliferation for both HEK293 cells and Human Umbilical Vein Endothelial Cells (HUVECs). These bioinks were shown to be dispensable by robotic precision, forming patterns and constructs that were insoluble and of suitable stiffness to endure post gelation handling. The two bioinks were further characterized for fabrication parameters and mechanical properties. The Author(s), 2015 Free form fabrication nationallicence Bioink nationallicence Transglutaminase nationallicence 3-D printing nationallicence Gelatin nationallicence Hydrogel nationallicence Irvine Scott Materials and Science Engineering, Division of Materials Technology, Nanyang Technological University, N4.1-01-30, 50 Nanyang Ave, 639798, Singapore, Singapore aut Agrawal Animesh Materials and Science Engineering, Division of Materials Technology, Nanyang Technological University, N4.1-01-30, 50 Nanyang Ave, 639798, Singapore, Singapore aut Lee Bae Materials and Science Engineering, Division of Materials Technology, Nanyang Technological University, N4.1-01-30, 50 Nanyang Ave, 639798, Singapore, Singapore aut Chua Hui Materials and Science Engineering, Division of Materials Technology, Nanyang Technological University, N4.1-01-30, 50 Nanyang Ave, 639798, Singapore, Singapore aut Low Kok Materials and Science Engineering, Division of Materials Technology, Nanyang Technological University, N4.1-01-30, 50 Nanyang Ave, 639798, Singapore, Singapore aut Lau Boon Materials and Science Engineering, Division of Materials Technology, Nanyang Technological University, N4.1-01-30, 50 Nanyang Ave, 639798, Singapore, Singapore aut Machluf Marcelle Faculty of Biotechnology and Food Engineering, Technion University, Haifa, Israel aut Venkatraman Subbu Materials and Science Engineering, Division of Materials Technology, Nanyang Technological University, N4.1-01-30, 50 Nanyang Ave, 639798, Singapore, Singapore aut Biomedical Microdevices Springer US; http://www.springer-ny.com 17/1(2015-02-01), 1-8 1387-2176 17:1<1 2015 17 10544 https://doi.org/10.1007/s10544-014-9915-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10544-014-9915-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Irvine Scott Materials and Science Engineering, Division of Materials Technology, Nanyang Technological University, N4.1-01-30, 50 Nanyang Ave, 639798, Singapore, Singapore aut NATIONALLICENCE 700 1- Agrawal Animesh Materials and Science Engineering, Division of Materials Technology, Nanyang Technological University, N4.1-01-30, 50 Nanyang Ave, 639798, Singapore, Singapore aut NATIONALLICENCE 700 1- Lee Bae Materials and Science Engineering, Division of Materials Technology, Nanyang Technological University, N4.1-01-30, 50 Nanyang Ave, 639798, Singapore, Singapore aut NATIONALLICENCE 700 1- Chua Hui Materials and Science Engineering, Division of Materials Technology, Nanyang Technological University, N4.1-01-30, 50 Nanyang Ave, 639798, Singapore, Singapore aut NATIONALLICENCE 700 1- Low Kok Materials and Science Engineering, Division of Materials Technology, Nanyang Technological University, N4.1-01-30, 50 Nanyang Ave, 639798, Singapore, Singapore aut NATIONALLICENCE 700 1- Lau Boon Materials and Science Engineering, Division of Materials Technology, Nanyang Technological University, N4.1-01-30, 50 Nanyang Ave, 639798, Singapore, Singapore aut NATIONALLICENCE 700 1- Machluf Marcelle Faculty of Biotechnology and Food Engineering, Technion University, Haifa, Israel aut NATIONALLICENCE 700 1- Venkatraman Subbu Materials and Science Engineering, Division of Materials Technology, Nanyang Technological University, N4.1-01-30, 50 Nanyang Ave, 639798, Singapore, Singapore aut NATIONALLICENCE 773 0- Biomedical Microdevices Springer US; http://www.springer-ny.com 17/1(2015-02-01), 1-8 1387-2176 17:1<1 2015 17 10544