Biocompatibility of Tygon® tubing in microfluidic cell culture
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| 024 | 7 | 0 | |a 10.1007/s10544-015-9938-9 |2 doi |
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| 245 | 0 | 0 | |a Biocompatibility of Tygon® tubing in microfluidic cell culture |h [Elektronische Daten] |c [Xiao Jiang, Rex Jeffries, Miguel Acosta, Andrey Tikunov, Jeffrey Macdonald, Glenn Walker, Michael Gamcsik] |
| 520 | 3 | |a Growth of the MDA-MB-231 breast cancer cell line in microfluidic channels was inhibited when culture media was delivered to the channels via microbore Tygon® tubing. Culture media incubated within this tubing also inhibited growth of these cells in conventional 96-well plates. These detrimental effects were not due to depletion of critical nutrients due to adsorption of media components onto the tubing surface. A pH change was also ruled out as a cause. Nuclear magnetic resonance spectroscopy of the cell growth media before and after incubation in the tubing confirmed no detectable loss of media components but did detect the presence of additional unidentified signals in the aliphatic region of the spectrum. These results indicate leaching of a chemical species from microbore Tygon® tubing that can affect cell growth in microfluidic devices. | |
| 540 | |a Springer Science+Business Media New York, 2015 | ||
| 690 | 7 | |a Biomaterial |2 nationallicence | |
| 690 | 7 | |a Cancer cell |2 nationallicence | |
| 690 | 7 | |a Biocompatibility |2 nationallicence | |
| 690 | 7 | |a Toxicity |2 nationallicence | |
| 690 | 7 | |a Polyvinylchloride |2 nationallicence | |
| 690 | 7 | |a Spectroscopy |2 nationallicence | |
| 700 | 1 | |a Jiang |D Xiao |u UNC/NCSU Joint Department of Biomedical Engineering, 4206D Engineering Building 3, 911 Oval Drive, 27695, Raleigh, NC, USA |4 aut | |
| 700 | 1 | |a Jeffries |D Rex |u UNC/NCSU Joint Department of Biomedical Engineering, 4206D Engineering Building 3, 911 Oval Drive, 27695, Raleigh, NC, USA |4 aut | |
| 700 | 1 | |a Acosta |D Miguel |u UNC/NCSU Joint Department of Biomedical Engineering, 4206D Engineering Building 3, 911 Oval Drive, 27695, Raleigh, NC, USA |4 aut | |
| 700 | 1 | |a Tikunov |D Andrey |u UNC/NCSU Joint Department of Biomedical Engineering, 4206D Engineering Building 3, 911 Oval Drive, 27695, Raleigh, NC, USA |4 aut | |
| 700 | 1 | |a Macdonald |D Jeffrey |u UNC/NCSU Joint Department of Biomedical Engineering, 4206D Engineering Building 3, 911 Oval Drive, 27695, Raleigh, NC, USA |4 aut | |
| 700 | 1 | |a Walker |D Glenn |u UNC/NCSU Joint Department of Biomedical Engineering, 4206D Engineering Building 3, 911 Oval Drive, 27695, Raleigh, NC, USA |4 aut | |
| 700 | 1 | |a Gamcsik |D Michael |u UNC/NCSU Joint Department of Biomedical Engineering, 4206D Engineering Building 3, 911 Oval Drive, 27695, Raleigh, NC, USA |4 aut | |
| 773 | 0 | |t Biomedical Microdevices |d Springer US; http://www.springer-ny.com |g 17/1(2015-02-01), 1-7 |x 1387-2176 |q 17:1<1 |1 2015 |2 17 |o 10544 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s10544-015-9938-9 |q text/html |z Onlinezugriff via DOI |
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| 908 | |D 1 |a research-article |2 jats | ||
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| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s10544-015-9938-9 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Jiang |D Xiao |u UNC/NCSU Joint Department of Biomedical Engineering, 4206D Engineering Building 3, 911 Oval Drive, 27695, Raleigh, NC, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Jeffries |D Rex |u UNC/NCSU Joint Department of Biomedical Engineering, 4206D Engineering Building 3, 911 Oval Drive, 27695, Raleigh, NC, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Acosta |D Miguel |u UNC/NCSU Joint Department of Biomedical Engineering, 4206D Engineering Building 3, 911 Oval Drive, 27695, Raleigh, NC, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Tikunov |D Andrey |u UNC/NCSU Joint Department of Biomedical Engineering, 4206D Engineering Building 3, 911 Oval Drive, 27695, Raleigh, NC, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Macdonald |D Jeffrey |u UNC/NCSU Joint Department of Biomedical Engineering, 4206D Engineering Building 3, 911 Oval Drive, 27695, Raleigh, NC, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Walker |D Glenn |u UNC/NCSU Joint Department of Biomedical Engineering, 4206D Engineering Building 3, 911 Oval Drive, 27695, Raleigh, NC, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Gamcsik |D Michael |u UNC/NCSU Joint Department of Biomedical Engineering, 4206D Engineering Building 3, 911 Oval Drive, 27695, Raleigh, NC, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Biomedical Microdevices |d Springer US; http://www.springer-ny.com |g 17/1(2015-02-01), 1-7 |x 1387-2176 |q 17:1<1 |1 2015 |2 17 |o 10544 | ||