caa a22 4500 60547933X CHVBK 20210128100409.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s10544-015-9938-9 doi (NATIONALLICENCE)springer-10.1007/s10544-015-9938-9 Biocompatibility of Tygon® tubing in microfluidic cell culture [Elektronische Daten] [Xiao Jiang, Rex Jeffries, Miguel Acosta, Andrey Tikunov, Jeffrey Macdonald, Glenn Walker, Michael Gamcsik] Growth of the MDA-MB-231 breast cancer cell line in microfluidic channels was inhibited when culture media was delivered to the channels via microbore Tygon® tubing. Culture media incubated within this tubing also inhibited growth of these cells in conventional 96-well plates. These detrimental effects were not due to depletion of critical nutrients due to adsorption of media components onto the tubing surface. A pH change was also ruled out as a cause. Nuclear magnetic resonance spectroscopy of the cell growth media before and after incubation in the tubing confirmed no detectable loss of media components but did detect the presence of additional unidentified signals in the aliphatic region of the spectrum. These results indicate leaching of a chemical species from microbore Tygon® tubing that can affect cell growth in microfluidic devices. Springer Science+Business Media New York, 2015 Biomaterial nationallicence Cancer cell nationallicence Biocompatibility nationallicence Toxicity nationallicence Polyvinylchloride nationallicence Spectroscopy nationallicence Jiang Xiao UNC/NCSU Joint Department of Biomedical Engineering, 4206D Engineering Building 3, 911 Oval Drive, 27695, Raleigh, NC, USA aut Jeffries Rex UNC/NCSU Joint Department of Biomedical Engineering, 4206D Engineering Building 3, 911 Oval Drive, 27695, Raleigh, NC, USA aut Acosta Miguel UNC/NCSU Joint Department of Biomedical Engineering, 4206D Engineering Building 3, 911 Oval Drive, 27695, Raleigh, NC, USA aut Tikunov Andrey UNC/NCSU Joint Department of Biomedical Engineering, 4206D Engineering Building 3, 911 Oval Drive, 27695, Raleigh, NC, USA aut Macdonald Jeffrey UNC/NCSU Joint Department of Biomedical Engineering, 4206D Engineering Building 3, 911 Oval Drive, 27695, Raleigh, NC, USA aut Walker Glenn UNC/NCSU Joint Department of Biomedical Engineering, 4206D Engineering Building 3, 911 Oval Drive, 27695, Raleigh, NC, USA aut Gamcsik Michael UNC/NCSU Joint Department of Biomedical Engineering, 4206D Engineering Building 3, 911 Oval Drive, 27695, Raleigh, NC, USA aut Biomedical Microdevices Springer US; http://www.springer-ny.com 17/1(2015-02-01), 1-7 1387-2176 17:1<1 2015 17 10544 https://doi.org/10.1007/s10544-015-9938-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10544-015-9938-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jiang Xiao UNC/NCSU Joint Department of Biomedical Engineering, 4206D Engineering Building 3, 911 Oval Drive, 27695, Raleigh, NC, USA aut NATIONALLICENCE 700 1- Jeffries Rex UNC/NCSU Joint Department of Biomedical Engineering, 4206D Engineering Building 3, 911 Oval Drive, 27695, Raleigh, NC, USA aut NATIONALLICENCE 700 1- Acosta Miguel UNC/NCSU Joint Department of Biomedical Engineering, 4206D Engineering Building 3, 911 Oval Drive, 27695, Raleigh, NC, USA aut NATIONALLICENCE 700 1- Tikunov Andrey UNC/NCSU Joint Department of Biomedical Engineering, 4206D Engineering Building 3, 911 Oval Drive, 27695, Raleigh, NC, USA aut NATIONALLICENCE 700 1- Macdonald Jeffrey UNC/NCSU Joint Department of Biomedical Engineering, 4206D Engineering Building 3, 911 Oval Drive, 27695, Raleigh, NC, USA aut NATIONALLICENCE 700 1- Walker Glenn UNC/NCSU Joint Department of Biomedical Engineering, 4206D Engineering Building 3, 911 Oval Drive, 27695, Raleigh, NC, USA aut NATIONALLICENCE 700 1- Gamcsik Michael UNC/NCSU Joint Department of Biomedical Engineering, 4206D Engineering Building 3, 911 Oval Drive, 27695, Raleigh, NC, USA aut NATIONALLICENCE 773 0- Biomedical Microdevices Springer US; http://www.springer-ny.com 17/1(2015-02-01), 1-7 1387-2176 17:1<1 2015 17 10544