caa a22 4500 605479348 CHVBK 20210128100409.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s10544-014-9905-x doi (NATIONALLICENCE)springer-10.1007/s10544-014-9905-x Microfluidic filtration and extraction of pathogens from food samples by hydrodynamic focusing and inertial lateral migration [Elektronische Daten] [Liviu Clime, Xuyen Hoa, Nathalie Corneau, Keith Morton, Christian Luebbert, Maxence Mounier, Daniel Brassard, Matthias Geissler, Sabah Bidawid, Jeff Farber, Teodor Veres] Detecting pathogenic bacteria in food or other biological samples with lab-on-a-chip (LOC) devices requires several sample preparation steps prior to analysis which commonly involves cleaning complex sample matrices of large debris. This often underestimated step is important to prevent these larger particles from clogging devices and to preserve initial concentrations when LOC techniques are used to concentrate or isolate smaller target microorganisms for downstream analysis. In this context, we developed a novel microfluidic system for membrane-free cleaning of biological samples from debris particles by combining hydrodynamic focusing and inertial lateral migration effects. The microfluidic device is fabricated using thermoplastic elastomers being compatible with thermoforming fabrication techniques leading to low-cost single-use devices. Microfluidic chip design and pumping protocols are optimized by investigating diffusive losses numerically with coupled Navier-Stokes and convective-diffusion theoretical models. Stability of inertial lateral migration and separation of debris is assessed through fluorescence microscopy measurements with labelled particles serving as a model system. Efficiency of debris cleaning is experimentally investigated by monitoring microchip outlets with in situ optical turbidity sensors, while retention of targeted pathogens (i.e., Listeria monocytogenes) within the sample stream is assessed through bacterial culture techniques. Optimized pumping protocols can remove up to 50% of debris from ground beef samples while percentage for preserved microorganisms can account for 95% in relatively clean samples. However, comparison between inoculated turbid and clean samples (i.e., with and without ground beef debris) indicate some degree of interference between debris inertial lateral migration and hydrodynamic focusing of small microorganisms. Although this interference can lead to significant decrease in chip performance through loss of target bacteria, it remains possible to reach 70% for sample recovery and more than 50% for debris removal even in the most turbid samples tested. Due to the relatively simple design, the robustness of the inertial migration effect itself, the high operational flow rates and fabrication methods that leverage low-cost materials, the proposed device can have an impact on a wide range of applications where high-throughput separation of particles and biological species is of interest. © Crown Copyright as represented by the National Research Council of Canada, 2015 Inertial microfluidics nationallicence Hydrodynamic focusing nationallicence Microfluidic filtration nationallicence Sample preparation nationallicence Hot embossing nationallicence Food safety inspection nationallicence Clime Liviu National Research Council of Canada, 75 Boulevard de Mortagne, J4B 6Y4, Boucherville, QC, Canada aut Hoa Xuyen National Research Council of Canada, 75 Boulevard de Mortagne, J4B 6Y4, Boucherville, QC, Canada aut Corneau Nathalie Bureau of Microbial Hazards, Health Canada, 251 Sir Frederick Banting Driveway, K1A 0K9, Ottawa, ON, Canada aut Morton Keith National Research Council of Canada, 75 Boulevard de Mortagne, J4B 6Y4, Boucherville, QC, Canada aut Luebbert Christian Bureau of Microbial Hazards, Health Canada, 251 Sir Frederick Banting Driveway, K1A 0K9, Ottawa, ON, Canada aut Mounier Maxence National Research Council of Canada, 75 Boulevard de Mortagne, J4B 6Y4, Boucherville, QC, Canada aut Brassard Daniel National Research Council of Canada, 75 Boulevard de Mortagne, J4B 6Y4, Boucherville, QC, Canada aut Geissler Matthias National Research Council of Canada, 75 Boulevard de Mortagne, J4B 6Y4, Boucherville, QC, Canada aut Bidawid Sabah Bureau of Microbial Hazards, Health Canada, 251 Sir Frederick Banting Driveway, K1A 0K9, Ottawa, ON, Canada aut Farber Jeff Bureau of Microbial Hazards, Health Canada, 251 Sir Frederick Banting Driveway, K1A 0K9, Ottawa, ON, Canada aut Veres Teodor National Research Council of Canada, 75 Boulevard de Mortagne, J4B 6Y4, Boucherville, QC, Canada aut Biomedical Microdevices Springer US; http://www.springer-ny.com 17/1(2015-02-01), 1-14 1387-2176 17:1<1 2015 17 10544 https://doi.org/10.1007/s10544-014-9905-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10544-014-9905-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Clime Liviu National Research Council of Canada, 75 Boulevard de Mortagne, J4B 6Y4, Boucherville, QC, Canada aut NATIONALLICENCE 700 1- Hoa Xuyen National Research Council of Canada, 75 Boulevard de Mortagne, J4B 6Y4, Boucherville, QC, Canada aut NATIONALLICENCE 700 1- Corneau Nathalie Bureau of Microbial Hazards, Health Canada, 251 Sir Frederick Banting Driveway, K1A 0K9, Ottawa, ON, Canada aut NATIONALLICENCE 700 1- Morton Keith National Research Council of Canada, 75 Boulevard de Mortagne, J4B 6Y4, Boucherville, QC, Canada aut NATIONALLICENCE 700 1- Luebbert Christian Bureau of Microbial Hazards, Health Canada, 251 Sir Frederick Banting Driveway, K1A 0K9, Ottawa, ON, Canada aut NATIONALLICENCE 700 1- Mounier Maxence National Research Council of Canada, 75 Boulevard de Mortagne, J4B 6Y4, Boucherville, QC, Canada aut NATIONALLICENCE 700 1- Brassard Daniel National Research Council of Canada, 75 Boulevard de Mortagne, J4B 6Y4, Boucherville, QC, Canada aut NATIONALLICENCE 700 1- Geissler Matthias National Research Council of Canada, 75 Boulevard de Mortagne, J4B 6Y4, Boucherville, QC, Canada aut NATIONALLICENCE 700 1- Bidawid Sabah Bureau of Microbial Hazards, Health Canada, 251 Sir Frederick Banting Driveway, K1A 0K9, Ottawa, ON, Canada aut NATIONALLICENCE 700 1- Farber Jeff Bureau of Microbial Hazards, Health Canada, 251 Sir Frederick Banting Driveway, K1A 0K9, Ottawa, ON, Canada aut NATIONALLICENCE 700 1- Veres Teodor National Research Council of Canada, 75 Boulevard de Mortagne, J4B 6Y4, Boucherville, QC, Canada aut NATIONALLICENCE 773 0- Biomedical Microdevices Springer US; http://www.springer-ny.com 17/1(2015-02-01), 1-14 1387-2176 17:1<1 2015 17 10544