Single channel and ensemble hERG conductance measured in droplet bilayers
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| 024 | 7 | 0 | |a 10.1007/s10544-014-9919-4 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s10544-014-9919-4 | ||
| 245 | 0 | 0 | |a Single channel and ensemble hERG conductance measured in droplet bilayers |h [Elektronische Daten] |c [Viksita Vijayvergiya, Shiv Acharya, Jason Poulos, Jacob Schmidt] |
| 520 | 3 | |a The human ether-a-go-go related gene (hERG) encodes the potassium channel Kv11.1, which plays a key role in the cardiac action potential and has been implicated in cardiac disorders as well as a number of off-target pharmaceutical interactions. The electrophysiology of this channel has been predominantly studied using patch clamp, but lipid bilayers have the potential to offer some advantages, including apparatus simplicity, ease of use, and the ability to control the membrane and solution compositions. We made membrane preparations from hERG-expressing cells and measured them using droplet bilayers, allowing measurement of channel ensemble currents and 13.5 pS single channel currents. These currents were ion selective and were blockable by E-4031 and dofetilide in a dose-dependent manner, allowing determination of IC50 values of 17nM and 9.65μM for E-4031 and dofetilide, respectively. We also observed time- and voltage- dependent currents following step changes in applied potential that were similar to previously reported patch clamp measurements. | |
| 540 | |a Springer Science+Business Media New York, 2015 | ||
| 690 | 7 | |a Ion channel conductance |2 nationallicence | |
| 690 | 7 | |a hERG |2 nationallicence | |
| 690 | 7 | |a Lipid bilayer |2 nationallicence | |
| 690 | 7 | |a Single channel |2 nationallicence | |
| 700 | 1 | |a Vijayvergiya |D Viksita |u Department of Bioengineering, University of California Los Angeles, 90095, Los Angeles, CA, USA |4 aut | |
| 700 | 1 | |a Acharya |D Shiv |u Department of Bioengineering, University of California Los Angeles, 90095, Los Angeles, CA, USA |4 aut | |
| 700 | 1 | |a Poulos |D Jason |u Librede Inc, Sherman Oaks, CA, USA |4 aut | |
| 700 | 1 | |a Schmidt |D Jacob |u Department of Bioengineering, University of California Los Angeles, 90095, Los Angeles, CA, USA |4 aut | |
| 773 | 0 | |t Biomedical Microdevices |d Springer US; http://www.springer-ny.com |g 17/1(2015-02-01), 1-7 |x 1387-2176 |q 17:1<1 |1 2015 |2 17 |o 10544 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s10544-014-9919-4 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s10544-014-9919-4 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Vijayvergiya |D Viksita |u Department of Bioengineering, University of California Los Angeles, 90095, Los Angeles, CA, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Acharya |D Shiv |u Department of Bioengineering, University of California Los Angeles, 90095, Los Angeles, CA, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Poulos |D Jason |u Librede Inc, Sherman Oaks, CA, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Schmidt |D Jacob |u Department of Bioengineering, University of California Los Angeles, 90095, Los Angeles, CA, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Biomedical Microdevices |d Springer US; http://www.springer-ny.com |g 17/1(2015-02-01), 1-7 |x 1387-2176 |q 17:1<1 |1 2015 |2 17 |o 10544 | ||