caa a22 4500 605479372 CHVBK 20210128100410.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s10544-014-9919-4 doi (NATIONALLICENCE)springer-10.1007/s10544-014-9919-4 Single channel and ensemble hERG conductance measured in droplet bilayers [Elektronische Daten] [Viksita Vijayvergiya, Shiv Acharya, Jason Poulos, Jacob Schmidt] The human ether-a-go-go related gene (hERG) encodes the potassium channel Kv11.1, which plays a key role in the cardiac action potential and has been implicated in cardiac disorders as well as a number of off-target pharmaceutical interactions. The electrophysiology of this channel has been predominantly studied using patch clamp, but lipid bilayers have the potential to offer some advantages, including apparatus simplicity, ease of use, and the ability to control the membrane and solution compositions. We made membrane preparations from hERG-expressing cells and measured them using droplet bilayers, allowing measurement of channel ensemble currents and 13.5 pS single channel currents. These currents were ion selective and were blockable by E-4031 and dofetilide in a dose-dependent manner, allowing determination of IC50 values of 17nM and 9.65μM for E-4031 and dofetilide, respectively. We also observed time- and voltage- dependent currents following step changes in applied potential that were similar to previously reported patch clamp measurements. Springer Science+Business Media New York, 2015 Ion channel conductance nationallicence hERG nationallicence Lipid bilayer nationallicence Single channel nationallicence Vijayvergiya Viksita Department of Bioengineering, University of California Los Angeles, 90095, Los Angeles, CA, USA aut Acharya Shiv Department of Bioengineering, University of California Los Angeles, 90095, Los Angeles, CA, USA aut Poulos Jason Librede Inc, Sherman Oaks, CA, USA aut Schmidt Jacob Department of Bioengineering, University of California Los Angeles, 90095, Los Angeles, CA, USA aut Biomedical Microdevices Springer US; http://www.springer-ny.com 17/1(2015-02-01), 1-7 1387-2176 17:1<1 2015 17 10544 https://doi.org/10.1007/s10544-014-9919-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10544-014-9919-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Vijayvergiya Viksita Department of Bioengineering, University of California Los Angeles, 90095, Los Angeles, CA, USA aut NATIONALLICENCE 700 1- Acharya Shiv Department of Bioengineering, University of California Los Angeles, 90095, Los Angeles, CA, USA aut NATIONALLICENCE 700 1- Poulos Jason Librede Inc, Sherman Oaks, CA, USA aut NATIONALLICENCE 700 1- Schmidt Jacob Department of Bioengineering, University of California Los Angeles, 90095, Los Angeles, CA, USA aut NATIONALLICENCE 773 0- Biomedical Microdevices Springer US; http://www.springer-ny.com 17/1(2015-02-01), 1-7 1387-2176 17:1<1 2015 17 10544