Balancing oxygen diffusion and convection in spiral microfluidics to mimic radial biological gradients
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| 024 | 7 | 0 | |a 10.1007/s10544-014-9922-9 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s10544-014-9922-9 | ||
| 245 | 0 | 0 | |a Balancing oxygen diffusion and convection in spiral microfluidics to mimic radial biological gradients |h [Elektronische Daten] |c [Zidong Li, Di Hu, Zhengtuo Zhao, Mengyang Zhou, Rui Liu, Joe Lo] |
| 520 | 3 | |a Biological gradients are more than linear, one-dimensional phenomena—they often manifest radial geometries superimposed over tissue features and in turn, elicit a spatial response. In wound healing, injury to tissue produces a hypoxic gradient towards the center of the wound, and wound cells respond to this by secreting growth hormones to promote healing. Despite this spatial element in tissue hypoxia, most in vitro hypoxia techniques rely on linear, diffusion-based gradients of limited dimensions. To demonstrate a large area, radial hypoxia gradient, a concentric spiral microfluidics was devised to balance oxygen diffusion against nitrogen convection. The devices were fabricated using only a simple robotic cutter and soft lithography. With these spirals, spatial gradients of 3-15% oxygen were delivered to fibroblast cells seeded across a gas-permeable membrane to modulate VEGF secretions. This technique opens the door for more studies on hypoxic gradients in wound healing and a number of tissue oxygen applications. | |
| 540 | |a Springer Science+Business Media New York, 2015 | ||
| 690 | 7 | |a Microfluidic |2 nationallicence | |
| 690 | 7 | |a Spatial gradient |2 nationallicence | |
| 690 | 7 | |a Oxygen |2 nationallicence | |
| 690 | 7 | |a Hypoxia |2 nationallicence | |
| 690 | 7 | |a Diffusion |2 nationallicence | |
| 690 | 7 | |a Convection |2 nationallicence | |
| 690 | 7 | |a Fibroblast |2 nationallicence | |
| 690 | 7 | |a VEGF secretion |2 nationallicence | |
| 700 | 1 | |a Li |D Zidong |u Bioengineering Program, Department of Mechanical Engineering, University of Michigan, HPEC 1332, 4901 Evergreen Rd., 48128, Dearborn, MI, USA |4 aut | |
| 700 | 1 | |a Hu |D Di |u Bioengineering Program, Department of Mechanical Engineering, University of Michigan, HPEC 1332, 4901 Evergreen Rd., 48128, Dearborn, MI, USA |4 aut | |
| 700 | 1 | |a Zhao |D Zhengtuo |u Bioengineering Program, Department of Mechanical Engineering, University of Michigan, HPEC 1332, 4901 Evergreen Rd., 48128, Dearborn, MI, USA |4 aut | |
| 700 | 1 | |a Zhou |D Mengyang |u Bioengineering Program, Department of Mechanical Engineering, University of Michigan, HPEC 1332, 4901 Evergreen Rd., 48128, Dearborn, MI, USA |4 aut | |
| 700 | 1 | |a Liu |D Rui |u Bioengineering Program, Department of Mechanical Engineering, University of Michigan, HPEC 1332, 4901 Evergreen Rd., 48128, Dearborn, MI, USA |4 aut | |
| 700 | 1 | |a Lo |D Joe |u Bioengineering Program, Department of Mechanical Engineering, University of Michigan, HPEC 1332, 4901 Evergreen Rd., 48128, Dearborn, MI, USA |4 aut | |
| 773 | 0 | |t Biomedical Microdevices |d Springer US; http://www.springer-ny.com |g 17/1(2015-02-01), 1-9 |x 1387-2176 |q 17:1<1 |1 2015 |2 17 |o 10544 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s10544-014-9922-9 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s10544-014-9922-9 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Li |D Zidong |u Bioengineering Program, Department of Mechanical Engineering, University of Michigan, HPEC 1332, 4901 Evergreen Rd., 48128, Dearborn, MI, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Hu |D Di |u Bioengineering Program, Department of Mechanical Engineering, University of Michigan, HPEC 1332, 4901 Evergreen Rd., 48128, Dearborn, MI, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Zhao |D Zhengtuo |u Bioengineering Program, Department of Mechanical Engineering, University of Michigan, HPEC 1332, 4901 Evergreen Rd., 48128, Dearborn, MI, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Zhou |D Mengyang |u Bioengineering Program, Department of Mechanical Engineering, University of Michigan, HPEC 1332, 4901 Evergreen Rd., 48128, Dearborn, MI, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Liu |D Rui |u Bioengineering Program, Department of Mechanical Engineering, University of Michigan, HPEC 1332, 4901 Evergreen Rd., 48128, Dearborn, MI, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Lo |D Joe |u Bioengineering Program, Department of Mechanical Engineering, University of Michigan, HPEC 1332, 4901 Evergreen Rd., 48128, Dearborn, MI, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Biomedical Microdevices |d Springer US; http://www.springer-ny.com |g 17/1(2015-02-01), 1-9 |x 1387-2176 |q 17:1<1 |1 2015 |2 17 |o 10544 | ||