caa a22 4500 605479410 CHVBK 20210128100410.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s10544-014-9911-z doi (NATIONALLICENCE)springer-10.1007/s10544-014-9911-z A nanostructured genosensor for the early diagnosis of systemic arterial hypertension [Elektronische Daten] [Thalita Rolim, Juliana Cancino, Valtencir Zucolotto] The rapid progress of nanomedicine, especially in areas related to medical imaging and diagnostics, has motivated the development of new nanomaterials that can be combined with biological materials for specific medical applications. One such area of research involves the detection of specific DNA sequences for the early diagnosis of genetic diseases, using nanoparticles-containing genosensors. Typical genosensors devices are based on the use of sensing electrodes - biorecognition platforms - containing immobilized capture DNA probes capable of hybridizing with specific target DNA sequences. In this paper we show that upon an appropriate design of the biorecognition platform, efficient sandwich-type genosensors based upon DNA-AuNPs nanocomplexes can be efficiently applied to the detection of a Systemic Arterial Hypertension (SAH) polymorphism located in intron 16 of the Angiotensin-converter enzyme (ACE) gene. Since SAH is intimately related to heart diseases, especially blood hypertension, its early detection is of great biomedical interest. The biorecognition platforms were assembled using mixed self-assembled monolayers (SAMmix), which provided the immobilization of organized architectures with molecular control. Detection of the DNA target sequence at concentrations down to 1nM was carried out using electrochemical impedance spectroscopy (EIS). We show that the use of EIS combined with specific nanobiocomplexes represents an efficient method for the unambiguous detection of complementary DNA hybridization for preventative nanomedicine applications. Springer Science+Business Media New York, 2015 Genosensor nationallicence Systemic arterial hypertension nationallicence Oligonucleotides nationallicence Nanocomplexes nationallicence Electrochemical impedance spectroscopy nationallicence Rolim Thalita Nanomedicine and Nanotoxicology Group, Physics Institute of São Carlos, University of São Paulo, CP 369, 13560-970, São Carlos, SP, Brazil aut Cancino Juliana Nanomedicine and Nanotoxicology Group, Physics Institute of São Carlos, University of São Paulo, CP 369, 13560-970, São Carlos, SP, Brazil aut Zucolotto Valtencir Nanomedicine and Nanotoxicology Group, Physics Institute of São Carlos, University of São Paulo, CP 369, 13560-970, São Carlos, SP, Brazil aut Biomedical Microdevices Springer US; http://www.springer-ny.com 17/1(2015-02-01), 1-9 1387-2176 17:1<1 2015 17 10544 https://doi.org/10.1007/s10544-014-9911-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10544-014-9911-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rolim Thalita Nanomedicine and Nanotoxicology Group, Physics Institute of São Carlos, University of São Paulo, CP 369, 13560-970, São Carlos, SP, Brazil aut NATIONALLICENCE 700 1- Cancino Juliana Nanomedicine and Nanotoxicology Group, Physics Institute of São Carlos, University of São Paulo, CP 369, 13560-970, São Carlos, SP, Brazil aut NATIONALLICENCE 700 1- Zucolotto Valtencir Nanomedicine and Nanotoxicology Group, Physics Institute of São Carlos, University of São Paulo, CP 369, 13560-970, São Carlos, SP, Brazil aut NATIONALLICENCE 773 0- Biomedical Microdevices Springer US; http://www.springer-ny.com 17/1(2015-02-01), 1-9 1387-2176 17:1<1 2015 17 10544