caa a22 4500 605479429 CHVBK 20210128100410.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s10544-014-9925-6 doi (NATIONALLICENCE)springer-10.1007/s10544-014-9925-6 A giant magnetoimpedance-based biosensor for sensitive detection of Escherichia coli O157:H7 [Elektronische Daten] [Zhen Yang, Xue-cheng Sun, Tao Wang, Chong Lei, Yan Liu, Yong Zhou, Jian Lei] A giant magnetoimpedance (GMI)-based biosensor was developed for detection of Escherichia coli (E. coli) O157: H7. The GMI sensor involving the sensing elements of Cr/Cu/NiFe/Cu/NiFe was fabricated by Micro Electro-Mechanical system (MEMS) technology, including thick photoresist lithography and electroplating. A separate Au film substrate as immunoplatform was used to capture E. coli O157:H7. The monoclonal mouse anti-E. coli antibody was immobilized on Au film substrate surface with a self-assembled layer. The different concentration E. coli O157:H7 (100, 300 and 500cfu/ml) were combined with Dynabeads-antibody conjugates (DAC) respectively. DAC were prepared by conjugating streptavidin-coupled Dynabeads with biotin-labeled polyclonal mouse anti-E. coli antibodies. The classical sandwich assay was used for detection of E. coli O157:H7 targeted with Dynabeads by using antibody-antigen pair combination of biotin-streptavidin. The fundamental principle for detection of E. coli O157:H7 based on GMI sensor was that Dynabeads were employed as magnetic labels of E. coli O157:H7, and E. coli O157:H7 can be monitored by detecting the fringe field of Dynabeads using magnetic sensing elements. We observed that the GMI ratio were significantly improved due to the presence of E. coli O157:H7 combined with Dynabeads. The GMI ratio increased as the E. coli O157:H7 concentration increased. A lower detectable concentration of 100cfu/ml was achieved in present work. The GMI-based biosensor provides a new method to rapid and sensitive detection E. coli O157:H7, which has a large potential for bio-application. Springer Science+Business Media New York, 2015 E. coli O157:H7 nationallicence Giant magnetoimpedance nationallicence Biosensor nationallicence Dynabeads nationallicence Au film nationallicence Yang Zhen Key Laboratory for Thin Film and Microfabrication of the Ministry of Education, Department of Micro/Nano Electronics, School of electronic information and electrical engineering, Shanghai Jiao Tong University, Dongchuan Road 800, 200240, Shanghai, People's Republic of China aut Sun Xue-cheng Key Laboratory for Thin Film and Microfabrication of the Ministry of Education, Department of Micro/Nano Electronics, School of electronic information and electrical engineering, Shanghai Jiao Tong University, Dongchuan Road 800, 200240, Shanghai, People's Republic of China aut Wang Tao Key Laboratory for Thin Film and Microfabrication of the Ministry of Education, Department of Micro/Nano Electronics, School of electronic information and electrical engineering, Shanghai Jiao Tong University, Dongchuan Road 800, 200240, Shanghai, People's Republic of China aut Lei Chong Key Laboratory for Thin Film and Microfabrication of the Ministry of Education, Department of Micro/Nano Electronics, School of electronic information and electrical engineering, Shanghai Jiao Tong University, Dongchuan Road 800, 200240, Shanghai, People's Republic of China aut Liu Yan Key Laboratory for Thin Film and Microfabrication of the Ministry of Education, Department of Micro/Nano Electronics, School of electronic information and electrical engineering, Shanghai Jiao Tong University, Dongchuan Road 800, 200240, Shanghai, People's Republic of China aut Zhou Yong Key Laboratory for Thin Film and Microfabrication of the Ministry of Education, Department of Micro/Nano Electronics, School of electronic information and electrical engineering, Shanghai Jiao Tong University, Dongchuan Road 800, 200240, Shanghai, People's Republic of China aut Lei Jian Key Laboratory for Thin Film and Microfabrication of the Ministry of Education, Department of Micro/Nano Electronics, School of electronic information and electrical engineering, Shanghai Jiao Tong University, Dongchuan Road 800, 200240, Shanghai, People's Republic of China aut Biomedical Microdevices Springer US; http://www.springer-ny.com 17/1(2015-02-01), 1-8 1387-2176 17:1<1 2015 17 10544 https://doi.org/10.1007/s10544-014-9925-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10544-014-9925-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yang Zhen Key Laboratory for Thin Film and Microfabrication of the Ministry of Education, Department of Micro/Nano Electronics, School of electronic information and electrical engineering, Shanghai Jiao Tong University, Dongchuan Road 800, 200240, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Sun Xue-cheng Key Laboratory for Thin Film and Microfabrication of the Ministry of Education, Department of Micro/Nano Electronics, School of electronic information and electrical engineering, Shanghai Jiao Tong University, Dongchuan Road 800, 200240, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Wang Tao Key Laboratory for Thin Film and Microfabrication of the Ministry of Education, Department of Micro/Nano Electronics, School of electronic information and electrical engineering, Shanghai Jiao Tong University, Dongchuan Road 800, 200240, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Lei Chong Key Laboratory for Thin Film and Microfabrication of the Ministry of Education, Department of Micro/Nano Electronics, School of electronic information and electrical engineering, Shanghai Jiao Tong University, Dongchuan Road 800, 200240, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Liu Yan Key Laboratory for Thin Film and Microfabrication of the Ministry of Education, Department of Micro/Nano Electronics, School of electronic information and electrical engineering, Shanghai Jiao Tong University, Dongchuan Road 800, 200240, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Zhou Yong Key Laboratory for Thin Film and Microfabrication of the Ministry of Education, Department of Micro/Nano Electronics, School of electronic information and electrical engineering, Shanghai Jiao Tong University, Dongchuan Road 800, 200240, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Lei Jian Key Laboratory for Thin Film and Microfabrication of the Ministry of Education, Department of Micro/Nano Electronics, School of electronic information and electrical engineering, Shanghai Jiao Tong University, Dongchuan Road 800, 200240, Shanghai, People's Republic of China aut NATIONALLICENCE 773 0- Biomedical Microdevices Springer US; http://www.springer-ny.com 17/1(2015-02-01), 1-8 1387-2176 17:1<1 2015 17 10544