caa a22 4500 605479445 CHVBK 20210128100410.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s10544-014-9920-y doi (NATIONALLICENCE)springer-10.1007/s10544-014-9920-y Rotary-based platform with disposable fluidic modules for automated isolation of nucleic acids [Elektronische Daten] [Dmitry Mamaev, Boris Shaskolskiy, Ekaterina Dementieva, Dmitry Khodakov, Dmitry Yurasov, Roman Yurasov, Danila Zimenkov, Vladimir Mikhailovich, Alexander Zasedatelev, Dmitry Gryadunov] We describe the development and evaluation of a rotary-based platform with multiple disposable fluidic modules for simultaneous automatic nucleic acid (NA) isolation from up to 24 biological samples. The procedure is performed inside insulated individual disposable modules, which minimizes both the risk of infection of personnel and laboratory cross-contamination. Each module is a segment of a circular cylinder containing a leak-proof inlet port for sample input, reservoirs with lyophilized chemicals and solvents, fluidic channels, stoppers, valves, a waste reservoir and an outlet port equipped with the standard micro test tube for NA collection. The entire platform, apart from the rotor that accommodates 24 modules, consists of functional elements that provide spinning of the rotor, reagent mixing, pressure delivery, and heating of reaction mixtures. The transfer of the reaction mixtures inside the modules is performed either with rotation of the rotor or with excessive air pressure applied to the module's reservoirs. The entire process takes less than 40min, starting from the sample loading to the recovery of the purified NA, and it allows NA isolation both from bacterial cells and viral particles. The feasibility and reproducibility of the developed platform was demonstrated by the NA isolation from suspensions of Bacillus thuringiensis and Mycobacterium tuberculosis cells within a concentration range of 108 to 102 cells/ml. Isolation of NAs from blood plasma samples with varying concentration of hepatitis B and C viruses from 107 to 102 particles/ml were also successful. The purity and integrity of the extracted NAs were both reliable for performing quantitative PCR. Springer Science+Business Media New York, 2015 Nucleic acids isolation nationallicence Fluidic module nationallicence Rotary-based platform nationallicence Workstation for automated nucleic acids isolation nationallicence Mamaev Dmitry Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 32 Vavilov Street, 119991, Moscow, Russia aut Shaskolskiy Boris Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 32 Vavilov Street, 119991, Moscow, Russia aut Dementieva Ekaterina Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 32 Vavilov Street, 119991, Moscow, Russia aut Khodakov Dmitry Flinders Centre for Nanoscale Science and Technology, School of Chemical and Physical Sciences, Flinders University, GPO Box 2100, 5001, Adelaide, SA, Australia aut Yurasov Dmitry Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 32 Vavilov Street, 119991, Moscow, Russia aut Yurasov Roman Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 32 Vavilov Street, 119991, Moscow, Russia aut Zimenkov Danila Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 32 Vavilov Street, 119991, Moscow, Russia aut Mikhailovich Vladimir Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 32 Vavilov Street, 119991, Moscow, Russia aut Zasedatelev Alexander Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 32 Vavilov Street, 119991, Moscow, Russia aut Gryadunov Dmitry Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 32 Vavilov Street, 119991, Moscow, Russia aut Biomedical Microdevices Springer US; http://www.springer-ny.com 17/1(2015-02-01), 1-12 1387-2176 17:1<1 2015 17 10544 https://doi.org/10.1007/s10544-014-9920-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10544-014-9920-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mamaev Dmitry Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 32 Vavilov Street, 119991, Moscow, Russia aut NATIONALLICENCE 700 1- Shaskolskiy Boris Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 32 Vavilov Street, 119991, Moscow, Russia aut NATIONALLICENCE 700 1- Dementieva Ekaterina Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 32 Vavilov Street, 119991, Moscow, Russia aut NATIONALLICENCE 700 1- Khodakov Dmitry Flinders Centre for Nanoscale Science and Technology, School of Chemical and Physical Sciences, Flinders University, GPO Box 2100, 5001, Adelaide, SA, Australia aut NATIONALLICENCE 700 1- Yurasov Dmitry Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 32 Vavilov Street, 119991, Moscow, Russia aut NATIONALLICENCE 700 1- Yurasov Roman Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 32 Vavilov Street, 119991, Moscow, Russia aut NATIONALLICENCE 700 1- Zimenkov Danila Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 32 Vavilov Street, 119991, Moscow, Russia aut NATIONALLICENCE 700 1- Mikhailovich Vladimir Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 32 Vavilov Street, 119991, Moscow, Russia aut NATIONALLICENCE 700 1- Zasedatelev Alexander Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 32 Vavilov Street, 119991, Moscow, Russia aut NATIONALLICENCE 700 1- Gryadunov Dmitry Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 32 Vavilov Street, 119991, Moscow, Russia aut NATIONALLICENCE 773 0- Biomedical Microdevices Springer US; http://www.springer-ny.com 17/1(2015-02-01), 1-12 1387-2176 17:1<1 2015 17 10544