caa a22 4500 605479461 CHVBK 20210128100410.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s10544-014-9907-8 doi (NATIONALLICENCE)springer-10.1007/s10544-014-9907-8 Multi-electrode array capable of supporting precisely patterned hippocampal neuronal networks [Elektronische Daten] [Tianyi Zhou, Susan Perry, Yevgeny Berdichevsky, Susanne Petryna, Vicki Fluck, Svetlana Tatic-Lucic] Accurate positioning of primary mouse hippocampal neurons on electrodes enables the recording from and stimulation of specified individual neurons on a multi-electrode array (MEA). In this work, positive dielectrophoresis (DEP) is applied to actively recruit hippocampal neurons to the electrodes of a MEA, whereas microstructures such as chambers and trenches are created to effectively define a patterned neuronal network. We present here the effective pretreatment methods, to improve cytocompatibility of cured thin SU-8 epoxy, commonly used in the fabrication of MEAs. The functionality of our novel MEA is proven by the successful recording of spontaneous and stimulated neuronal potentials from primary hippocampal neurons, including the propagation of evoked neuronal bursts between electrodes. Springer Science+Business Media New York, 2015 Multi-electrode array (MEA) nationallicence Dielectrophoresis (DEP) nationallicence Precise patterning nationallicence SU-8 cytocompatibility nationallicence Hippocampal neuronal network nationallicence Neuronal recording nationallicence Zhou Tianyi Department of Electrical and Computer Engineering, Lehigh University, 18015, Bethlehem, PA, USA aut Perry Susan Bioengineering Program, Lehigh University, 18015, Bethlehem, PA, USA aut Berdichevsky Yevgeny Department of Electrical and Computer Engineering, Lehigh University, 18015, Bethlehem, PA, USA aut Petryna Susanne Bioengineering Program, Lehigh University, 18015, Bethlehem, PA, USA aut Fluck Vicki Bioengineering Program, Lehigh University, 18015, Bethlehem, PA, USA aut Tatic-Lucic Svetlana Department of Electrical and Computer Engineering, Lehigh University, 18015, Bethlehem, PA, USA aut Biomedical Microdevices Springer US; http://www.springer-ny.com 17/1(2015-02-01), 1-12 1387-2176 17:1<1 2015 17 10544 https://doi.org/10.1007/s10544-014-9907-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10544-014-9907-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhou Tianyi Department of Electrical and Computer Engineering, Lehigh University, 18015, Bethlehem, PA, USA aut NATIONALLICENCE 700 1- Perry Susan Bioengineering Program, Lehigh University, 18015, Bethlehem, PA, USA aut NATIONALLICENCE 700 1- Berdichevsky Yevgeny Department of Electrical and Computer Engineering, Lehigh University, 18015, Bethlehem, PA, USA aut NATIONALLICENCE 700 1- Petryna Susanne Bioengineering Program, Lehigh University, 18015, Bethlehem, PA, USA aut NATIONALLICENCE 700 1- Fluck Vicki Bioengineering Program, Lehigh University, 18015, Bethlehem, PA, USA aut NATIONALLICENCE 700 1- Tatic-Lucic Svetlana Department of Electrical and Computer Engineering, Lehigh University, 18015, Bethlehem, PA, USA aut NATIONALLICENCE 773 0- Biomedical Microdevices Springer US; http://www.springer-ny.com 17/1(2015-02-01), 1-12 1387-2176 17:1<1 2015 17 10544