caa a22 4500 605479488 CHVBK 20210128100410.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s10544-015-0007-1 doi (NATIONALLICENCE)springer-10.1007/s10544-015-0007-1 Sub-population analysis of deformability distribution in heterogeneous red blood cell population [Elektronische Daten] [Dong Lee, Il Doh, Frans Kuypers, Young-Ho Cho] We present a method for sub-population analysis of deformability distribution using single-cell microchamber array (SiCMA) technology. It is a unique method allowing the correlation of overall cellular characteristics with surface and cytosolic characteristics to define the distribution of individual cellular characteristics in heterogeneous cell populations. As a proof of principle, reticulocytes, the immature sub-population of red blood cells (RBC), were recognized from RBC population by a surface marker and different characteristics on deformability between these populations were characterized. The proposed technology can be used in a variety of applications that would benefit from the ability to measure the distribution of cellular characteristics in complex populations, especially important to define hematologic disorders. Springer Science+Business Media New York, 2015 Single cell analysis nationallicence Deformability distribution nationallicence Sub-population analysis nationallicence Microchamber array nationallicence Lee Dong Central R&D Institute, Samsung Electro-mechanics Co., Ltd., Suwon, Republic of Korea aut Doh Il Center for Medical Metrology, Korea Research Institute of Standards and Science (KRISS), Daejeon, Republic of Korea aut Kuypers Frans Department of Hematology, Children's Hospital Oakland Research Institute, Oakland, CA, USA aut Cho Young-Ho Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Republic of Korea aut Biomedical Microdevices Springer US; http://www.springer-ny.com 17/6(2015-12-01), 1-5 1387-2176 17:6<1 2015 17 10544 https://doi.org/10.1007/s10544-015-0007-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10544-015-0007-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lee Dong Central R&D Institute, Samsung Electro-mechanics Co., Ltd., Suwon, Republic of Korea aut NATIONALLICENCE 700 1- Doh Il Center for Medical Metrology, Korea Research Institute of Standards and Science (KRISS), Daejeon, Republic of Korea aut NATIONALLICENCE 700 1- Kuypers Frans Department of Hematology, Children's Hospital Oakland Research Institute, Oakland, CA, USA aut NATIONALLICENCE 700 1- Cho Young-Ho Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Republic of Korea aut NATIONALLICENCE 773 0- Biomedical Microdevices Springer US; http://www.springer-ny.com 17/6(2015-12-01), 1-5 1387-2176 17:6<1 2015 17 10544