caa a22 4500 605479496 CHVBK 20210128100410.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s10544-015-0017-z doi (NATIONALLICENCE)springer-10.1007/s10544-015-0017-z Development of a microfluidic device for cell concentration and blood cell-plasma separation [Elektronische Daten] [M. Maria, B. Kumar, T. Chandra, A. Sen] This work presents design, fabrication and test of a microfluidic device which employs Fahraeus-Lindqvist and Zweifach-Fung effects for cell concentration and blood cell-plasma separation. The device design comprises a straight main channel with a series of branched channels placed symmetrically on both sides of the main channel. The design implements constrictions before each junction (branching point) in order to direct cells that would have migrated closer to the wall (naturally or after liquid extraction at a junction) towards the centre of the main channel. Theoretical and numerical analysis are performed for design of the microchannel network to ensure that a minimum flow rate ratio (of 2.5:1, main channel-to-side channels) is maintained at each junction and predict flow rate at the plasma outlet. The dimensions and location of the constrictions were determined using numerical simulations. The effect of presence of constrictions before the junctions was demonstrated by comparing the performances of the device with and without constrictions. To demonstrate the performance of the device, initial experiments were performed with polystyrene microbeads (10 and 15μm size) and droplets. Finally, the device was used for concentration of HL60 cells and separation of plasma and cells in diluted blood samples. The cell concentration and blood-plasma purification efficiency was quantified using Haemocytometer and Fluorescence-Activated Cell Sorter (FACS). A seven-fold cell concentration was obtained with HL60 cells and a purification efficiency of 70% and plasma recovery of 80% was observed for diluted (1:20) blood sample. FACS was used to identify cell lysis and the cell viability was checked using Trypan Blue test which showed that more than 99% cells are alive indicating the suitability of the device for practical use. The proposed device has potential to be used as a sample preparation module in lab on chip based diagnostic platforms. Springer Science+Business Media New York, 2015 Microfluidics nationallicence Cell-plasma separation nationallicence Passive separation nationallicence Maria M. Department of Mechanical Engineering, Indian Institute of Technology Madras, 600036, Chennai, India aut Kumar B. Department of Mechanical Engineering, Indian Institute of Technology Madras, 600036, Chennai, India aut Chandra T. Department of Biotechnology, Indian Institute of Technology Madras, 600036, Chennai, India aut Sen A. Department of Mechanical Engineering, Indian Institute of Technology Madras, 600036, Chennai, India aut Biomedical Microdevices Springer US; http://www.springer-ny.com 17/6(2015-12-01), 1-19 1387-2176 17:6<1 2015 17 10544 https://doi.org/10.1007/s10544-015-0017-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10544-015-0017-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Maria M. Department of Mechanical Engineering, Indian Institute of Technology Madras, 600036, Chennai, India aut NATIONALLICENCE 700 1- Kumar B. Department of Mechanical Engineering, Indian Institute of Technology Madras, 600036, Chennai, India aut NATIONALLICENCE 700 1- Chandra T. Department of Biotechnology, Indian Institute of Technology Madras, 600036, Chennai, India aut NATIONALLICENCE 700 1- Sen A. Department of Mechanical Engineering, Indian Institute of Technology Madras, 600036, Chennai, India aut NATIONALLICENCE 773 0- Biomedical Microdevices Springer US; http://www.springer-ny.com 17/6(2015-12-01), 1-19 1387-2176 17:6<1 2015 17 10544