caa a22 4500 60547950X CHVBK 20210128100410.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s10544-015-0002-6 doi (NATIONALLICENCE)springer-10.1007/s10544-015-0002-6 A model microfluidics-based system for the human and mouse retina [Elektronische Daten] [Shawn Mishra, Ankush Thakur, Stephen Redenti, Maribel Vazquez] The application of microfluidics technologies to the study of retinal function and response holds great promise for development of new and improved treatments for patients with degenerative retinal diseases. Restoration of vision via retinal transplantation therapy has been severely limited by the low numbers of motile cells observed post transplantation. Using modern soft lithographic techniques, we have developed the μRetina, a novel and convenient biomimetic microfluidics device capable of examing the migratory behavior of retinal lineage cells within biomimetic geometries of the human and mouse retina. Coupled computer simulations and experimental validations were used to characterize and confirm the formation of chemical concentration gradients within the μRetina, while real-time images within the device captured radial and theta cell migration in response to concentration gradients of stromal derived factor (SDF-1), a known chemoattractant. Our data underscore how the μRetina can be used to examine the concentration-dependent migration of retinal progenitors in order to enhance current therapies, as well as develop novel migration-targeted treatments. Springer Science+Business Media New York, 2015 Retina nationallicence Progenitor nationallicence SDF-1 nationallicence Diffusion nationallicence Migration nationallicence Mishra Shawn Department of Biomedical Engineering, City College of New York, New York, NY, USA aut Thakur Ankush Department of Biomedical Engineering, City College of New York, New York, NY, USA aut Redenti Stephen Department of Biology, Lehman College, New York, NY, USA aut Vazquez Maribel Department of Biomedical Engineering, City College of New York, New York, NY, USA aut Biomedical Microdevices Springer US; http://www.springer-ny.com 17/6(2015-12-01), 1-10 1387-2176 17:6<1 2015 17 10544 https://doi.org/10.1007/s10544-015-0002-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10544-015-0002-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mishra Shawn Department of Biomedical Engineering, City College of New York, New York, NY, USA aut NATIONALLICENCE 700 1- Thakur Ankush Department of Biomedical Engineering, City College of New York, New York, NY, USA aut NATIONALLICENCE 700 1- Redenti Stephen Department of Biology, Lehman College, New York, NY, USA aut NATIONALLICENCE 700 1- Vazquez Maribel Department of Biomedical Engineering, City College of New York, New York, NY, USA aut NATIONALLICENCE 773 0- Biomedical Microdevices Springer US; http://www.springer-ny.com 17/6(2015-12-01), 1-10 1387-2176 17:6<1 2015 17 10544