caa a22 4500 605479534 CHVBK 20210128100410.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s10544-015-0015-1 doi (NATIONALLICENCE)springer-10.1007/s10544-015-0015-1 Microfluidic emulation of mechanical circulatory support device shear-mediated platelet activation [Elektronische Daten] [Annalisa Dimasi, Marco Rasponi, Jawaad Sheriff, Wei-Che Chiu, Danny Bluestein, Phat Tran, Marvin Slepian, Alberto Redaelli] Thrombosis of ventricular assist devices (VADs) compromises their performance, with associated risks of systemic embolization, stroke, pump stop and possible death. Anti-thrombotic (AT) drugs, utilized to limit thrombosis, are largely dosed empirically, with limited testing of their efficacy. Further, such testing, if performed, typically examines efficacy under static conditions, which is not reflective of actual shear-mediated flow. Here we adopted our previously developed Device Thrombogenicity Emulation methodology to design microfluidic platforms able to emulate representative shear stress profiles of mechanical circulatory support (MCS) devices. Our long-term goal is to utilize these systems for point-of-care (POC) personalized testing of AT efficacy under specific, individual shear profiles. First, we designed different types of microfluidic channels able to replicate sample shear stress patterns observed in MCS devices. Second, we explored the flexibility of microfluidic technology in generating dynamic shear stress profiles by modulating the geometrical features of the channels. Finally, we designed microfluidic channel systems able to emulate the shear stress profiles of two commercial VADs. From CFD analyses, the VAD-emulating microfluidic systems were able to replicate the main characteristics of the shear stress waveforms of the macroscale VADs (i.e., shear stress peaks and duration). Our results establish the basis for development of a lab-on-chip POC system able to perform device-specific and patient-specific platelet activation state assays. Springer Science+Business Media New York, 2015 Mechanical circulatory support nationallicence Ventricular assist devices nationallicence Thrombosis nationallicence Microfluidics nationallicence Computational fluid dynamics nationallicence Anti-thrombotic therapy nationallicence Dimasi Annalisa Department of Electronics, Information and Bioengineering, Politecnico di Milano, 20133, Milan, Italy aut Rasponi Marco Department of Electronics, Information and Bioengineering, Politecnico di Milano, 20133, Milan, Italy aut Sheriff Jawaad Department of Biomedical Engineering, Stony Brook University, 11794-8151, Stony Brook, NY, USA aut Chiu Wei-Che Department of Biomedical Engineering, Stony Brook University, 11794-8151, Stony Brook, NY, USA aut Bluestein Danny Department of Biomedical Engineering, Stony Brook University, 11794-8151, Stony Brook, NY, USA aut Tran Phat Department of Medicine and Biomedical Engineering, Sarver Heart Center, University of Arizona, 1501 North Campbell Avenue, 85724, Tucson, AZ, USA aut Slepian Marvin Department of Biomedical Engineering, Stony Brook University, 11794-8151, Stony Brook, NY, USA aut Redaelli Alberto Department of Electronics, Information and Bioengineering, Politecnico di Milano, 20133, Milan, Italy aut Biomedical Microdevices Springer US; http://www.springer-ny.com 17/6(2015-12-01), 1-11 1387-2176 17:6<1 2015 17 10544 https://doi.org/10.1007/s10544-015-0015-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10544-015-0015-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dimasi Annalisa Department of Electronics, Information and Bioengineering, Politecnico di Milano, 20133, Milan, Italy aut NATIONALLICENCE 700 1- Rasponi Marco Department of Electronics, Information and Bioengineering, Politecnico di Milano, 20133, Milan, Italy aut NATIONALLICENCE 700 1- Sheriff Jawaad Department of Biomedical Engineering, Stony Brook University, 11794-8151, Stony Brook, NY, USA aut NATIONALLICENCE 700 1- Chiu Wei-Che Department of Biomedical Engineering, Stony Brook University, 11794-8151, Stony Brook, NY, USA aut NATIONALLICENCE 700 1- Bluestein Danny Department of Biomedical Engineering, Stony Brook University, 11794-8151, Stony Brook, NY, USA aut NATIONALLICENCE 700 1- Tran Phat Department of Medicine and Biomedical Engineering, Sarver Heart Center, University of Arizona, 1501 North Campbell Avenue, 85724, Tucson, AZ, USA aut NATIONALLICENCE 700 1- Slepian Marvin Department of Biomedical Engineering, Stony Brook University, 11794-8151, Stony Brook, NY, USA aut NATIONALLICENCE 700 1- Redaelli Alberto Department of Electronics, Information and Bioengineering, Politecnico di Milano, 20133, Milan, Italy aut NATIONALLICENCE 773 0- Biomedical Microdevices Springer US; http://www.springer-ny.com 17/6(2015-12-01), 1-11 1387-2176 17:6<1 2015 17 10544