Effect of microfabricated microgroove-surface devices on the morphology of mesenchymal stem cells
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| 024 | 7 | 0 | |a 10.1007/s10544-015-0016-0 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s10544-015-0016-0 | ||
| 245 | 0 | 0 | |a Effect of microfabricated microgroove-surface devices on the morphology of mesenchymal stem cells |h [Elektronische Daten] |c [Xiangkai Zhang, Tomoki Aoyama, Takashi Yasuda, Makoto Oike, Akira Ito, Junichi Tajino, Momoko Nagai, Rune Fujioka, Hirotaka Iijima, Shoki Yamaguchi, Norihiro Kakinuma, Hiroshi Kuroki] |
| 520 | 3 | |a The surface of a material that is in contact with cells is known to affect cell morphology and function. To develop an appropriate surface for tendon engineering, we used zigzag microgroove surfaces, which are similar to the tenocyte microenvironment. The purpose of this study was to investigate the effect of microgroove surfaces with different ridge angles (RAs), ridge lengths (RLs), ridge widths (RWs), and groove widths (GWs) on human bone marrow-derived mesenchymal stem cell (MSC) shape. Dishes with microgroove surfaces were fabricated using cyclic olefin polymer by injection-compression molding. The other parameters were fixed, and effects of different RAs (180 - 30 °), RLs (5 - 500μm), RWs (5 - 500μm), and GWs (5 - 500μm) were examined. Changes in the zigzag shape of the cell due to different RAs, RLs, RWs, and GWs were observed by optical microscopy and scanning electron microscopy. Cytoskeletal changes were investigated using Phalloidin immunofluorescence staining. As observed by optical microscopy, MSCs changed to a zigzag shape in response to microgroove surfaces with different ridge and groove properties. . As observed by scanning electron microscopy, the cell shape changed at turns in the microgroove surface. Phalloidin immunofluorescence staining indicated that F-actin, not only in cell filopodia but also inside the cell body, changed orientation to conform to the microgrooves. In conclusion, the use of zigzag microgroove surfaces microfabricated by injection-compression molding demonstrated the property of MSCs to alter their shapes to fit the surface. | |
| 540 | |a Springer Science+Business Media New York, 2015 | ||
| 690 | 7 | |a Mesenchymal stem cell |2 nationallicence | |
| 690 | 7 | |a Microgroove |2 nationallicence | |
| 690 | 7 | |a Cell shape |2 nationallicence | |
| 690 | 7 | |a Zigzag |2 nationallicence | |
| 700 | 1 | |a Zhang |D Xiangkai |u Human Health Sciences, Graduate School of Medicine, Kyoto University, 53 kawahara-cho, Shogoin, 606-8507, Sakyo-ku, Kyoto, Japan |4 aut | |
| 700 | 1 | |a Aoyama |D Tomoki |u Human Health Sciences, Graduate School of Medicine, Kyoto University, 53 kawahara-cho, Shogoin, 606-8507, Sakyo-ku, Kyoto, Japan |4 aut | |
| 700 | 1 | |a Yasuda |D Takashi |u Precision Machinery Department, SEIKOH GIKEN Co., Ltd., Chiba, Japan |4 aut | |
| 700 | 1 | |a Oike |D Makoto |u Precision Machinery Department, SEIKOH GIKEN Co., Ltd., Chiba, Japan |4 aut | |
| 700 | 1 | |a Ito |D Akira |u Human Health Sciences, Graduate School of Medicine, Kyoto University, 53 kawahara-cho, Shogoin, 606-8507, Sakyo-ku, Kyoto, Japan |4 aut | |
| 700 | 1 | |a Tajino |D Junichi |u Human Health Sciences, Graduate School of Medicine, Kyoto University, 53 kawahara-cho, Shogoin, 606-8507, Sakyo-ku, Kyoto, Japan |4 aut | |
| 700 | 1 | |a Nagai |D Momoko |u Human Health Sciences, Graduate School of Medicine, Kyoto University, 53 kawahara-cho, Shogoin, 606-8507, Sakyo-ku, Kyoto, Japan |4 aut | |
| 700 | 1 | |a Fujioka |D Rune |u Human Health Sciences, Graduate School of Medicine, Kyoto University, 53 kawahara-cho, Shogoin, 606-8507, Sakyo-ku, Kyoto, Japan |4 aut | |
| 700 | 1 | |a Iijima |D Hirotaka |u Human Health Sciences, Graduate School of Medicine, Kyoto University, 53 kawahara-cho, Shogoin, 606-8507, Sakyo-ku, Kyoto, Japan |4 aut | |
| 700 | 1 | |a Yamaguchi |D Shoki |u Human Health Sciences, Graduate School of Medicine, Kyoto University, 53 kawahara-cho, Shogoin, 606-8507, Sakyo-ku, Kyoto, Japan |4 aut | |
| 700 | 1 | |a Kakinuma |D Norihiro |u Precision Machinery Department, SEIKOH GIKEN Co., Ltd., Chiba, Japan |4 aut | |
| 700 | 1 | |a Kuroki |D Hiroshi |u Human Health Sciences, Graduate School of Medicine, Kyoto University, 53 kawahara-cho, Shogoin, 606-8507, Sakyo-ku, Kyoto, Japan |4 aut | |
| 773 | 0 | |t Biomedical Microdevices |d Springer US; http://www.springer-ny.com |g 17/6(2015-12-01), 1-8 |x 1387-2176 |q 17:6<1 |1 2015 |2 17 |o 10544 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s10544-015-0016-0 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s10544-015-0016-0 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Zhang |D Xiangkai |u Human Health Sciences, Graduate School of Medicine, Kyoto University, 53 kawahara-cho, Shogoin, 606-8507, Sakyo-ku, Kyoto, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Aoyama |D Tomoki |u Human Health Sciences, Graduate School of Medicine, Kyoto University, 53 kawahara-cho, Shogoin, 606-8507, Sakyo-ku, Kyoto, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Yasuda |D Takashi |u Precision Machinery Department, SEIKOH GIKEN Co., Ltd., Chiba, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Oike |D Makoto |u Precision Machinery Department, SEIKOH GIKEN Co., Ltd., Chiba, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Ito |D Akira |u Human Health Sciences, Graduate School of Medicine, Kyoto University, 53 kawahara-cho, Shogoin, 606-8507, Sakyo-ku, Kyoto, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Tajino |D Junichi |u Human Health Sciences, Graduate School of Medicine, Kyoto University, 53 kawahara-cho, Shogoin, 606-8507, Sakyo-ku, Kyoto, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Nagai |D Momoko |u Human Health Sciences, Graduate School of Medicine, Kyoto University, 53 kawahara-cho, Shogoin, 606-8507, Sakyo-ku, Kyoto, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Fujioka |D Rune |u Human Health Sciences, Graduate School of Medicine, Kyoto University, 53 kawahara-cho, Shogoin, 606-8507, Sakyo-ku, Kyoto, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Iijima |D Hirotaka |u Human Health Sciences, Graduate School of Medicine, Kyoto University, 53 kawahara-cho, Shogoin, 606-8507, Sakyo-ku, Kyoto, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Yamaguchi |D Shoki |u Human Health Sciences, Graduate School of Medicine, Kyoto University, 53 kawahara-cho, Shogoin, 606-8507, Sakyo-ku, Kyoto, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Kakinuma |D Norihiro |u Precision Machinery Department, SEIKOH GIKEN Co., Ltd., Chiba, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Kuroki |D Hiroshi |u Human Health Sciences, Graduate School of Medicine, Kyoto University, 53 kawahara-cho, Shogoin, 606-8507, Sakyo-ku, Kyoto, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Biomedical Microdevices |d Springer US; http://www.springer-ny.com |g 17/6(2015-12-01), 1-8 |x 1387-2176 |q 17:6<1 |1 2015 |2 17 |o 10544 | ||