caa a22 4500 605479542 CHVBK 20210128100410.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s10544-015-0016-0 doi (NATIONALLICENCE)springer-10.1007/s10544-015-0016-0 Effect of microfabricated microgroove-surface devices on the morphology of mesenchymal stem cells [Elektronische Daten] [Xiangkai Zhang, Tomoki Aoyama, Takashi Yasuda, Makoto Oike, Akira Ito, Junichi Tajino, Momoko Nagai, Rune Fujioka, Hirotaka Iijima, Shoki Yamaguchi, Norihiro Kakinuma, Hiroshi Kuroki] The surface of a material that is in contact with cells is known to affect cell morphology and function. To develop an appropriate surface for tendon engineering, we used zigzag microgroove surfaces, which are similar to the tenocyte microenvironment. The purpose of this study was to investigate the effect of microgroove surfaces with different ridge angles (RAs), ridge lengths (RLs), ridge widths (RWs), and groove widths (GWs) on human bone marrow-derived mesenchymal stem cell (MSC) shape. Dishes with microgroove surfaces were fabricated using cyclic olefin polymer by injection-compression molding. The other parameters were fixed, and effects of different RAs (180 - 30 °), RLs (5 - 500μm), RWs (5 - 500μm), and GWs (5 - 500μm) were examined. Changes in the zigzag shape of the cell due to different RAs, RLs, RWs, and GWs were observed by optical microscopy and scanning electron microscopy. Cytoskeletal changes were investigated using Phalloidin immunofluorescence staining. As observed by optical microscopy, MSCs changed to a zigzag shape in response to microgroove surfaces with different ridge and groove properties. . As observed by scanning electron microscopy, the cell shape changed at turns in the microgroove surface. Phalloidin immunofluorescence staining indicated that F-actin, not only in cell filopodia but also inside the cell body, changed orientation to conform to the microgrooves. In conclusion, the use of zigzag microgroove surfaces microfabricated by injection-compression molding demonstrated the property of MSCs to alter their shapes to fit the surface. Springer Science+Business Media New York, 2015 Mesenchymal stem cell nationallicence Microgroove nationallicence Cell shape nationallicence Zigzag nationallicence Zhang Xiangkai Human Health Sciences, Graduate School of Medicine, Kyoto University, 53 kawahara-cho, Shogoin, 606-8507, Sakyo-ku, Kyoto, Japan aut Aoyama Tomoki Human Health Sciences, Graduate School of Medicine, Kyoto University, 53 kawahara-cho, Shogoin, 606-8507, Sakyo-ku, Kyoto, Japan aut Yasuda Takashi Precision Machinery Department, SEIKOH GIKEN Co., Ltd., Chiba, Japan aut Oike Makoto Precision Machinery Department, SEIKOH GIKEN Co., Ltd., Chiba, Japan aut Ito Akira Human Health Sciences, Graduate School of Medicine, Kyoto University, 53 kawahara-cho, Shogoin, 606-8507, Sakyo-ku, Kyoto, Japan aut Tajino Junichi Human Health Sciences, Graduate School of Medicine, Kyoto University, 53 kawahara-cho, Shogoin, 606-8507, Sakyo-ku, Kyoto, Japan aut Nagai Momoko Human Health Sciences, Graduate School of Medicine, Kyoto University, 53 kawahara-cho, Shogoin, 606-8507, Sakyo-ku, Kyoto, Japan aut Fujioka Rune Human Health Sciences, Graduate School of Medicine, Kyoto University, 53 kawahara-cho, Shogoin, 606-8507, Sakyo-ku, Kyoto, Japan aut Iijima Hirotaka Human Health Sciences, Graduate School of Medicine, Kyoto University, 53 kawahara-cho, Shogoin, 606-8507, Sakyo-ku, Kyoto, Japan aut Yamaguchi Shoki Human Health Sciences, Graduate School of Medicine, Kyoto University, 53 kawahara-cho, Shogoin, 606-8507, Sakyo-ku, Kyoto, Japan aut Kakinuma Norihiro Precision Machinery Department, SEIKOH GIKEN Co., Ltd., Chiba, Japan aut Kuroki Hiroshi Human Health Sciences, Graduate School of Medicine, Kyoto University, 53 kawahara-cho, Shogoin, 606-8507, Sakyo-ku, Kyoto, Japan aut Biomedical Microdevices Springer US; http://www.springer-ny.com 17/6(2015-12-01), 1-8 1387-2176 17:6<1 2015 17 10544 https://doi.org/10.1007/s10544-015-0016-0 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10544-015-0016-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang Xiangkai Human Health Sciences, Graduate School of Medicine, Kyoto University, 53 kawahara-cho, Shogoin, 606-8507, Sakyo-ku, Kyoto, Japan aut NATIONALLICENCE 700 1- Aoyama Tomoki Human Health Sciences, Graduate School of Medicine, Kyoto University, 53 kawahara-cho, Shogoin, 606-8507, Sakyo-ku, Kyoto, Japan aut NATIONALLICENCE 700 1- Yasuda Takashi Precision Machinery Department, SEIKOH GIKEN Co., Ltd., Chiba, Japan aut NATIONALLICENCE 700 1- Oike Makoto Precision Machinery Department, SEIKOH GIKEN Co., Ltd., Chiba, Japan aut NATIONALLICENCE 700 1- Ito Akira Human Health Sciences, Graduate School of Medicine, Kyoto University, 53 kawahara-cho, Shogoin, 606-8507, Sakyo-ku, Kyoto, Japan aut NATIONALLICENCE 700 1- Tajino Junichi Human Health Sciences, Graduate School of Medicine, Kyoto University, 53 kawahara-cho, Shogoin, 606-8507, Sakyo-ku, Kyoto, Japan aut NATIONALLICENCE 700 1- Nagai Momoko Human Health Sciences, Graduate School of Medicine, Kyoto University, 53 kawahara-cho, Shogoin, 606-8507, Sakyo-ku, Kyoto, Japan aut NATIONALLICENCE 700 1- Fujioka Rune Human Health Sciences, Graduate School of Medicine, Kyoto University, 53 kawahara-cho, Shogoin, 606-8507, Sakyo-ku, Kyoto, Japan aut NATIONALLICENCE 700 1- Iijima Hirotaka Human Health Sciences, Graduate School of Medicine, Kyoto University, 53 kawahara-cho, Shogoin, 606-8507, Sakyo-ku, Kyoto, Japan aut NATIONALLICENCE 700 1- Yamaguchi Shoki Human Health Sciences, Graduate School of Medicine, Kyoto University, 53 kawahara-cho, Shogoin, 606-8507, Sakyo-ku, Kyoto, Japan aut NATIONALLICENCE 700 1- Kakinuma Norihiro Precision Machinery Department, SEIKOH GIKEN Co., Ltd., Chiba, Japan aut NATIONALLICENCE 700 1- Kuroki Hiroshi Human Health Sciences, Graduate School of Medicine, Kyoto University, 53 kawahara-cho, Shogoin, 606-8507, Sakyo-ku, Kyoto, Japan aut NATIONALLICENCE 773 0- Biomedical Microdevices Springer US; http://www.springer-ny.com 17/6(2015-12-01), 1-8 1387-2176 17:6<1 2015 17 10544