caa a22 4500 605479550 CHVBK 20210128100410.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s10544-015-0013-3 doi (NATIONALLICENCE)springer-10.1007/s10544-015-0013-3 Selective pattern of cancer cell accumulation and growth using UV modulating printing of hydrogels [Elektronische Daten] [Wenguang Yang, Haibo Yu, Fanan Wei, Gongxin Li, Yuechao Wang, Lianqing Liu] Fabrication of extracellular microenvironment for cancer cell growth in vitro is an indispensable technique to precisely control the cell spatial arrangement and proliferation for cell-behavior research. Current micropatterning methods usually require relatively complicated operations, which makes it difficult to investigate the effects of different cell growth patterns. This manuscript proposes a DMD-based projection technique to quickly pattern a poly(ethylene) glycol diacrylate (PEGDA)-based hydrogel on a common glass substrate. Using this method, we can effectively control the growth patterns of cells. Compared with these traditional methods which employ digital dynamic mask, polymerization of PEGDA solution can be used to create arbitrary shaped microstructures with high efficiency, flexibility and repeatability. The duration of UV exposure is less than 10s through controlling the projected illumination pattern. The ability of patterned PEGDA-coated film to hinder cell adhesion makes it possible to control area over which cells attach. In our experiments, we take advantage of the blank area to pattern cells, which allows cells to grow in various pre-designed shapes and sizes. And the patterning cells have a high viability after culturing for several days. Interestingly, we found that the restricted space could stiffen and strengthen the cells. These results indicate that cells and extracellular microenvironment can influence each other. Springer Science+Business Media New York, 2015 Cell pattern nationallicence Microfabrication nationallicence Bioengineering nationallicence Bioprinting nationallicence Yang Wenguang State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang, People's Republic of China aut Yu Haibo State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang, People's Republic of China aut Wei Fanan State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang, People's Republic of China aut Li Gongxin State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang, People's Republic of China aut Wang Yuechao State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang, People's Republic of China aut Liu Lianqing State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang, People's Republic of China aut Biomedical Microdevices Springer US; http://www.springer-ny.com 17/6(2015-12-01), 1-8 1387-2176 17:6<1 2015 17 10544 https://doi.org/10.1007/s10544-015-0013-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10544-015-0013-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yang Wenguang State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang, People's Republic of China aut NATIONALLICENCE 700 1- Yu Haibo State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang, People's Republic of China aut NATIONALLICENCE 700 1- Wei Fanan State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang, People's Republic of China aut NATIONALLICENCE 700 1- Li Gongxin State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang, People's Republic of China aut NATIONALLICENCE 700 1- Wang Yuechao State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang, People's Republic of China aut NATIONALLICENCE 700 1- Liu Lianqing State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang, People's Republic of China aut NATIONALLICENCE 773 0- Biomedical Microdevices Springer US; http://www.springer-ny.com 17/6(2015-12-01), 1-8 1387-2176 17:6<1 2015 17 10544