caa a22 4500 605479569 CHVBK 20210128100410.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s10544-015-0012-4 doi (NATIONALLICENCE)springer-10.1007/s10544-015-0012-4 Handcrafted multilayer PDMS microchannel scaffolds for peripheral nerve regeneration [Elektronische Daten] [Ridwan Hossain, Bongkyun Kim, Rachel Pankratz, Ali Ajam, Sungreol Park, Sibani Biswal, Yoonsu Choi] Injuries that result in the loss of limb functionality may be caused by the severing of the peripheral nerves within the affected limb. Several bioengineered peripheral nerve scaffolds have been developed in order to provide the physical support and topographical guidance necessary for the naturally disorganized axon outgrowth to reattach to distal nerve stumps as an alternative to other procedures, like nerve grafting. PDMS has been chosen for the base material of the scaffolds due to its biocompatibility, flexibility, transparency, and well-developed fabrication techniques. The process of observing the axon outgrowth across the nerve gaps with PDMS scaffolds has been challenging due to the limited number and fineness of longitudinal sections that can be extracted from harvested nerve tissue samples after implantation. To address this, multilayer microchannel scaffolds were developed with the object of providing more refined longitudinal observation of axon outgrowth by longitudinally ‘sectioning' the device during fabrication, removing the need for much of the sample preparation process. This device was then implanted into the sciatic nerves of Lewis rats, and then harvested after two and four weeks to analyze the difference in nerve regeneration between two different time periods. The present layer by layer structure, which is separable after nerve regeneration and is treated as an individual layer during the histology process, provides the details of biological events during axonal regeneration. Confocal microscopic imaging showed the details of peripheral nerve regeneration including nerve branches and growth cones observable from within the microchannels of the multilayer PDMS microchannel scaffolds. Springer Science+Business Media New York, 2015 Peripheral nerve regeneration nationallicence Microchannel nationallicence Immunohistochemistry nationallicence Neural interface nationallicence Hossain Ridwan Department of Electrical Engineering, University of Texas - Rio Grande Valley, Edinburg, TX, USA aut Kim Bongkyun Department of Electrical Engineering, University of Texas - Rio Grande Valley, Edinburg, TX, USA aut Pankratz Rachel Department of Electrical Engineering, University of Texas - Rio Grande Valley, Edinburg, TX, USA aut Ajam Ali Department of Electrical Engineering, University of Texas - Rio Grande Valley, Edinburg, TX, USA aut Park Sungreol Department of Electrical Engineering, University of Texas - Rio Grande Valley, Edinburg, TX, USA aut Biswal Sibani Department of Chemical and Biomolecular Engineering, Rice University, Houston, TX, USA aut Choi Yoonsu Department of Electrical Engineering, University of Texas - Rio Grande Valley, Edinburg, TX, USA aut Biomedical Microdevices Springer US; http://www.springer-ny.com 17/6(2015-12-01), 1-9 1387-2176 17:6<1 2015 17 10544 https://doi.org/10.1007/s10544-015-0012-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10544-015-0012-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hossain Ridwan Department of Electrical Engineering, University of Texas - Rio Grande Valley, Edinburg, TX, USA aut NATIONALLICENCE 700 1- Kim Bongkyun Department of Electrical Engineering, University of Texas - Rio Grande Valley, Edinburg, TX, USA aut NATIONALLICENCE 700 1- Pankratz Rachel Department of Electrical Engineering, University of Texas - Rio Grande Valley, Edinburg, TX, USA aut NATIONALLICENCE 700 1- Ajam Ali Department of Electrical Engineering, University of Texas - Rio Grande Valley, Edinburg, TX, USA aut NATIONALLICENCE 700 1- Park Sungreol Department of Electrical Engineering, University of Texas - Rio Grande Valley, Edinburg, TX, USA aut NATIONALLICENCE 700 1- Biswal Sibani Department of Chemical and Biomolecular Engineering, Rice University, Houston, TX, USA aut NATIONALLICENCE 700 1- Choi Yoonsu Department of Electrical Engineering, University of Texas - Rio Grande Valley, Edinburg, TX, USA aut NATIONALLICENCE 773 0- Biomedical Microdevices Springer US; http://www.springer-ny.com 17/6(2015-12-01), 1-9 1387-2176 17:6<1 2015 17 10544