A complementary method to CD4 counting: measurement of CD4+/CD8+ T lymphocyte ratio in a tandem affinity microfluidic system
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| 024 | 7 | 0 | |a 10.1007/s10544-015-0023-1 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s10544-015-0023-1 | ||
| 245 | 0 | 2 | |a A complementary method to CD4 counting: measurement of CD4+/CD8+ T lymphocyte ratio in a tandem affinity microfluidic system |h [Elektronische Daten] |c [Wenjie Li, Yan Gao, Dimitri Pappas] |
| 520 | 3 | |a We describe a tandem affinity microfluidic separation that measures the ratio of CD4+/CD8+ T lymphocytes from blood samples. It is performed by injecting 2μL of lysed blood samples at 1800-2700cellsμL-1 into a microfluidic device containing two serially linked affinity regions, followed with a stop flow incubation that captures CD4+/CD8+ T lymphocytes on the corresponding affinity regions. Fluorophore conjugated antibodies are then injected at a controlled shear stress of 1.7dyn cm-2 to label target cells while eluting non-specific cells; and at last the CD4/CD8 ratio is calculated after the cell enumeration. The ratio of CD4+/CD8+ T lymphocytes achieved by our tandem affinity microfluidic system was in close agreement with that performed using conventional flow cytometry (R 2 = 0.97) over a wide range (0.4-2.5) that covered the reference values from immune deficient patients to healthy people. This approach may represent an inexpensive and powerful tool in diagnosis of immunodeficiency disorders including HIV or mycobacterium tuberculosis. | |
| 540 | |a Springer Science+Business Media New York, 2015 | ||
| 690 | 7 | |a Tandem affinity microfluidic separation |2 nationallicence | |
| 690 | 7 | |a Blood cells |2 nationallicence | |
| 690 | 7 | |a CD4+/CD8 + T lymphocytes |2 nationallicence | |
| 690 | 7 | |a CD4/CD8 ratio |2 nationallicence | |
| 690 | 7 | |a Cytometry |2 nationallicence | |
| 700 | 1 | |a Li |D Wenjie |u Department of Chemistry and Biochemistry, Texas Tech University, 79409, Lubbock, TX, USA |4 aut | |
| 700 | 1 | |a Gao |D Yan |u Department of Chemistry and Biochemistry, Texas Tech University, 79409, Lubbock, TX, USA |4 aut | |
| 700 | 1 | |a Pappas |D Dimitri |u Department of Chemistry and Biochemistry, Texas Tech University, 79409, Lubbock, TX, USA |4 aut | |
| 773 | 0 | |t Biomedical Microdevices |d Springer US; http://www.springer-ny.com |g 17/6(2015-12-01), 1-9 |x 1387-2176 |q 17:6<1 |1 2015 |2 17 |o 10544 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s10544-015-0023-1 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s10544-015-0023-1 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Li |D Wenjie |u Department of Chemistry and Biochemistry, Texas Tech University, 79409, Lubbock, TX, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Gao |D Yan |u Department of Chemistry and Biochemistry, Texas Tech University, 79409, Lubbock, TX, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Pappas |D Dimitri |u Department of Chemistry and Biochemistry, Texas Tech University, 79409, Lubbock, TX, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Biomedical Microdevices |d Springer US; http://www.springer-ny.com |g 17/6(2015-12-01), 1-9 |x 1387-2176 |q 17:6<1 |1 2015 |2 17 |o 10544 | ||