Retina-on-a-chip: a microfluidic platform for point access signaling studies
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| 024 | 7 | 0 | |a 10.1007/s10544-015-0019-x |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s10544-015-0019-x | ||
| 245 | 0 | 0 | |a Retina-on-a-chip: a microfluidic platform for point access signaling studies |h [Elektronische Daten] |c [Kirsten Dodson, Franklin Echevarria, Deyu Li, Rebecca Sappington, Jon Edd] |
| 520 | 3 | |a We report on a microfluidic platform for culture of whole organs or tissue slices with the capability of point access reagent delivery to probe the transport of signaling events. Whole mice retina were maintained for multiple days with negative pressure applied to tightly but gently bind the bottom of the retina to a thin poly-(dimethylsiloxane) membrane, through which twelve 100μm diameter through-holes served as fluidic access points. Staining with toluidine blue, transport of locally applied cholera toxin beta, and transient response to lipopolysaccharide in the retina demonstrated the capability of the microfluidic platform. The point access fluidic delivery capability could enable new assays in the study of various kinds of excised tissues, including retina. | |
| 540 | |a Springer Science+Business Media New York, 2015 | ||
| 690 | 7 | |a Microfluidic tissue culture |2 nationallicence | |
| 690 | 7 | |a Retina |2 nationallicence | |
| 690 | 7 | |a Microenvironment |2 nationallicence | |
| 700 | 1 | |a Dodson |D Kirsten |u Department of Mechanical Engineering, Vanderbilt University, Nashville, TN, USA |4 aut | |
| 700 | 1 | |a Echevarria |D Franklin |u Department of Ophthalmology and Visual Sciences, Vanderbilt Eye Institute, Vanderbilt University School of Medicine, Nashville, TN, USA |4 aut | |
| 700 | 1 | |a Li |D Deyu |u Department of Mechanical Engineering, Vanderbilt University, Nashville, TN, USA |4 aut | |
| 700 | 1 | |a Sappington |D Rebecca |u Department of Ophthalmology and Visual Sciences, Vanderbilt Eye Institute, Vanderbilt University School of Medicine, Nashville, TN, USA |4 aut | |
| 700 | 1 | |a Edd |D Jon |u Department of Mechanical Engineering, Vanderbilt University, Nashville, TN, USA |4 aut | |
| 773 | 0 | |t Biomedical Microdevices |d Springer US; http://www.springer-ny.com |g 17/6(2015-12-01), 1-10 |x 1387-2176 |q 17:6<1 |1 2015 |2 17 |o 10544 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s10544-015-0019-x |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s10544-015-0019-x |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Dodson |D Kirsten |u Department of Mechanical Engineering, Vanderbilt University, Nashville, TN, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Echevarria |D Franklin |u Department of Ophthalmology and Visual Sciences, Vanderbilt Eye Institute, Vanderbilt University School of Medicine, Nashville, TN, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Li |D Deyu |u Department of Mechanical Engineering, Vanderbilt University, Nashville, TN, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Sappington |D Rebecca |u Department of Ophthalmology and Visual Sciences, Vanderbilt Eye Institute, Vanderbilt University School of Medicine, Nashville, TN, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Edd |D Jon |u Department of Mechanical Engineering, Vanderbilt University, Nashville, TN, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Biomedical Microdevices |d Springer US; http://www.springer-ny.com |g 17/6(2015-12-01), 1-10 |x 1387-2176 |q 17:6<1 |1 2015 |2 17 |o 10544 | ||