caa a22 4500 605479585 CHVBK 20210128100410.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s10544-015-0019-x doi (NATIONALLICENCE)springer-10.1007/s10544-015-0019-x Retina-on-a-chip: a microfluidic platform for point access signaling studies [Elektronische Daten] [Kirsten Dodson, Franklin Echevarria, Deyu Li, Rebecca Sappington, Jon Edd] We report on a microfluidic platform for culture of whole organs or tissue slices with the capability of point access reagent delivery to probe the transport of signaling events. Whole mice retina were maintained for multiple days with negative pressure applied to tightly but gently bind the bottom of the retina to a thin poly-(dimethylsiloxane) membrane, through which twelve 100μm diameter through-holes served as fluidic access points. Staining with toluidine blue, transport of locally applied cholera toxin beta, and transient response to lipopolysaccharide in the retina demonstrated the capability of the microfluidic platform. The point access fluidic delivery capability could enable new assays in the study of various kinds of excised tissues, including retina. Springer Science+Business Media New York, 2015 Microfluidic tissue culture nationallicence Retina nationallicence Microenvironment nationallicence Dodson Kirsten Department of Mechanical Engineering, Vanderbilt University, Nashville, TN, USA aut Echevarria Franklin Department of Ophthalmology and Visual Sciences, Vanderbilt Eye Institute, Vanderbilt University School of Medicine, Nashville, TN, USA aut Li Deyu Department of Mechanical Engineering, Vanderbilt University, Nashville, TN, USA aut Sappington Rebecca Department of Ophthalmology and Visual Sciences, Vanderbilt Eye Institute, Vanderbilt University School of Medicine, Nashville, TN, USA aut Edd Jon Department of Mechanical Engineering, Vanderbilt University, Nashville, TN, USA aut Biomedical Microdevices Springer US; http://www.springer-ny.com 17/6(2015-12-01), 1-10 1387-2176 17:6<1 2015 17 10544 https://doi.org/10.1007/s10544-015-0019-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10544-015-0019-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dodson Kirsten Department of Mechanical Engineering, Vanderbilt University, Nashville, TN, USA aut NATIONALLICENCE 700 1- Echevarria Franklin Department of Ophthalmology and Visual Sciences, Vanderbilt Eye Institute, Vanderbilt University School of Medicine, Nashville, TN, USA aut NATIONALLICENCE 700 1- Li Deyu Department of Mechanical Engineering, Vanderbilt University, Nashville, TN, USA aut NATIONALLICENCE 700 1- Sappington Rebecca Department of Ophthalmology and Visual Sciences, Vanderbilt Eye Institute, Vanderbilt University School of Medicine, Nashville, TN, USA aut NATIONALLICENCE 700 1- Edd Jon Department of Mechanical Engineering, Vanderbilt University, Nashville, TN, USA aut NATIONALLICENCE 773 0- Biomedical Microdevices Springer US; http://www.springer-ny.com 17/6(2015-12-01), 1-10 1387-2176 17:6<1 2015 17 10544