caa a22 4500 605479593 CHVBK 20210128100411.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s10544-015-0021-3 doi (NATIONALLICENCE)springer-10.1007/s10544-015-0021-3 Isolation of nucleated red blood cells in maternal blood for Non-invasive prenatal diagnosis [Elektronische Daten] [Yeongje Byeon, Chang-Seok Ki, Ki-Ho Han] This paper introduces a two-step cascade enrichment method for isolating nucleated red blood cells (NRBCs) in maternal blood. The two-step enrichment platform consists of a positive enrichment process based on a red blood cell (RBC) hyperaggregation method and a negative enrichment process using microfluidic technology. An analytical evaluation using blood samples from patients with leukemia showed that the while blood cell (WBC) depletion and NRBC loss rates of the positive enrichment process were 93.98% and 6.02%, respectively. Through the two-step cascade enrichment method, 1-396 NRBCs and only 0-6 WBCs were isolated from 1mL of 18 maternal blood samples. Experimental results also showed that the WBC depletion rate of the proposed two-step method was more than 625,000-fold, and the purity of enriched NRBCs ranged from 20% to 100%. Furthermore, SRY (the sex-determining region of the Y chromosome) genes were detected in enriched NRBCs, thereby demonstrating that enriched NRBCs contain fetus-derived NRBCs. Springer Science+Business Media New York, 2015 Nucleated red blood cells nationallicence Prenatal diagnosis nationallicence RBC hyperaggregation nationallicence Lateral magnetophoretic microseparator nationallicence Microfluidic device nationallicence Byeon Yeongje Department of Nano Science and Engineering, Center for Nano Manufacturing, Inje University, 607 Obang-dong, 621-749, Gimhae, Gyongnam, Republic of Korea aut Ki Chang-Seok Department of Laboratory Medicine and Genetics, Samsung Medical Center, Sungkyunkwan University School of Medicine, 81 Irwon-ro, 135-710, Gangnam-gu, Seoul, Republic of Korea aut Han Ki-Ho Department of Nano Science and Engineering, Center for Nano Manufacturing, Inje University, 607 Obang-dong, 621-749, Gimhae, Gyongnam, Republic of Korea aut Biomedical Microdevices Springer US; http://www.springer-ny.com 17/6(2015-12-01), 1-7 1387-2176 17:6<1 2015 17 10544 https://doi.org/10.1007/s10544-015-0021-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10544-015-0021-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Byeon Yeongje Department of Nano Science and Engineering, Center for Nano Manufacturing, Inje University, 607 Obang-dong, 621-749, Gimhae, Gyongnam, Republic of Korea aut NATIONALLICENCE 700 1- Ki Chang-Seok Department of Laboratory Medicine and Genetics, Samsung Medical Center, Sungkyunkwan University School of Medicine, 81 Irwon-ro, 135-710, Gangnam-gu, Seoul, Republic of Korea aut NATIONALLICENCE 700 1- Han Ki-Ho Department of Nano Science and Engineering, Center for Nano Manufacturing, Inje University, 607 Obang-dong, 621-749, Gimhae, Gyongnam, Republic of Korea aut NATIONALLICENCE 773 0- Biomedical Microdevices Springer US; http://www.springer-ny.com 17/6(2015-12-01), 1-7 1387-2176 17:6<1 2015 17 10544