caa a22 4500 605479607 CHVBK 20210128100411.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s10544-015-0010-6 doi (NATIONALLICENCE)springer-10.1007/s10544-015-0010-6 Fabrication of uniform-sized poly-ɛ-caprolactone microspheres and their applications in human embryonic stem cell culture [Elektronische Daten] [Jian Li, Alan Lam, Jessica Toh, Shaul Reuveny, Steve Oh, William Birch] The generation of liquefied poly-ɛ-caprolactone (PCL) droplets by means of a microfluidic device results in uniform-sized microspheres, which are validated as microcarriers for human embryonic stem cell culture. Formed droplet size and size distribution, as well as the resulting PCL microsphere size, are correlated with the viscosity and flow rate ratio of the dispersed (Q d) and continuous (Q c) phases. PCL in dichloromethane increases its viscosity with concentration and molecular weight. Higher viscosity and Q d/Q c lead to the formation of larger droplets, within two observed formation modes: dripping and jetting. At low viscosity of dispersed phase and Q d/Q c, the microfluidic device is operated in dripping mode, which generates droplets and microspheres with greater size uniformity. Solutions with lower molecular weight PCL have lower viscosity, resulting in a wider concentration range for the dripping mode. When coated with extracellular matrix (ECM) proteins, the fabricated PCL microspheres are demonstrated capable of supporting the expansion of human embryonic stem cells. Springer Science+Business Media New York, 2015 Polymer microspheres nationallicence Microfluidics nationallicence Poly-ɛ-caprolactone nationallicence Microcarriers nationallicence Human embryonic stem cells nationallicence Li Jian A*STAR (Agency for Science, Technology and Research), Institute of Materials Research and Engineering, 2 Fusionopolis Way, Innovis, #08-03, 138634, Singapore, Singapore aut Lam Alan A*STAR (Agency for Science, Technology and Research), Bioprocessing Technology Institute, 20 Biopolis Way, #06-01, 138668, Singapore, Singapore aut Toh Jessica A*STAR (Agency for Science, Technology and Research), Institute of Materials Research and Engineering, 2 Fusionopolis Way, Innovis, #08-03, 138634, Singapore, Singapore aut Reuveny Shaul A*STAR (Agency for Science, Technology and Research), Bioprocessing Technology Institute, 20 Biopolis Way, #06-01, 138668, Singapore, Singapore aut Oh Steve A*STAR (Agency for Science, Technology and Research), Bioprocessing Technology Institute, 20 Biopolis Way, #06-01, 138668, Singapore, Singapore aut Birch William A*STAR (Agency for Science, Technology and Research), Institute of Materials Research and Engineering, 2 Fusionopolis Way, Innovis, #08-03, 138634, Singapore, Singapore aut Biomedical Microdevices Springer US; http://www.springer-ny.com 17/6(2015-12-01), 1-12 1387-2176 17:6<1 2015 17 10544 https://doi.org/10.1007/s10544-015-0010-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10544-015-0010-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li Jian A*STAR (Agency for Science, Technology and Research), Institute of Materials Research and Engineering, 2 Fusionopolis Way, Innovis, #08-03, 138634, Singapore, Singapore aut NATIONALLICENCE 700 1- Lam Alan A*STAR (Agency for Science, Technology and Research), Bioprocessing Technology Institute, 20 Biopolis Way, #06-01, 138668, Singapore, Singapore aut NATIONALLICENCE 700 1- Toh Jessica A*STAR (Agency for Science, Technology and Research), Institute of Materials Research and Engineering, 2 Fusionopolis Way, Innovis, #08-03, 138634, Singapore, Singapore aut NATIONALLICENCE 700 1- Reuveny Shaul A*STAR (Agency for Science, Technology and Research), Bioprocessing Technology Institute, 20 Biopolis Way, #06-01, 138668, Singapore, Singapore aut NATIONALLICENCE 700 1- Oh Steve A*STAR (Agency for Science, Technology and Research), Bioprocessing Technology Institute, 20 Biopolis Way, #06-01, 138668, Singapore, Singapore aut NATIONALLICENCE 700 1- Birch William A*STAR (Agency for Science, Technology and Research), Institute of Materials Research and Engineering, 2 Fusionopolis Way, Innovis, #08-03, 138634, Singapore, Singapore aut NATIONALLICENCE 773 0- Biomedical Microdevices Springer US; http://www.springer-ny.com 17/6(2015-12-01), 1-12 1387-2176 17:6<1 2015 17 10544