High-throughput blood cell focusing and plasma isolation using spiral inertial microfluidic devices
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| 008 | 210128e20151201xx s 000 0 eng | ||
| 024 | 7 | 0 | |a 10.1007/s10544-015-0018-y |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s10544-015-0018-y | ||
| 245 | 0 | 0 | |a High-throughput blood cell focusing and plasma isolation using spiral inertial microfluidic devices |h [Elektronische Daten] |c [Nan Xiang, Zhonghua Ni] |
| 520 | 3 | |a Herein, we explored the blood cell focusing and plasma isolation using a spiral inertial microfluidic device. First, the flow-rate and concentration effects on the migration dynamics of blood cells were systematically investigated to uncover the focusing mechanisms and steric crowding effects of cells in Dean-coupled inertial flows. A novel phenomenon that the focusing status of discoid red blood cells (RBCs) changes according to the channel height was discovered. These experimental data may provide valuable insights for the high-throughput processing of blood samples using inertial microfluidics. On the basis of the improved understandings on blood cell focusing, efficient isolation of plasma from whole blood with a 20-fold dilution was achieved at a throughput up to 700μl/min. The purity of the isolated blood plasma was close to 100%, and the plasma yield was calculated to be 38.5%. As compared with previously-reported devices, our spiral inertial microfluidic device provides a balanced overall performance, and has overriding advantages in terms of processing throughput and operating efficiency. | |
| 540 | |a Springer Science+Business Media New York, 2015 | ||
| 690 | 7 | |a Blood cell focusing |2 nationallicence | |
| 690 | 7 | |a Plasma isolation |2 nationallicence | |
| 690 | 7 | |a Inertial microfluidics |2 nationallicence | |
| 690 | 7 | |a High-throughput processing |2 nationallicence | |
| 690 | 7 | |a Migration dynamics |2 nationallicence | |
| 700 | 1 | |a Xiang |D Nan |u School of Mechanical Engineering, and Jiangsu Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments, Southeast University, 211189, Nanjing, China |4 aut | |
| 700 | 1 | |a Ni |D Zhonghua |u School of Mechanical Engineering, and Jiangsu Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments, Southeast University, 211189, Nanjing, China |4 aut | |
| 773 | 0 | |t Biomedical Microdevices |d Springer US; http://www.springer-ny.com |g 17/6(2015-12-01), 1-11 |x 1387-2176 |q 17:6<1 |1 2015 |2 17 |o 10544 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s10544-015-0018-y |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s10544-015-0018-y |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Xiang |D Nan |u School of Mechanical Engineering, and Jiangsu Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments, Southeast University, 211189, Nanjing, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Ni |D Zhonghua |u School of Mechanical Engineering, and Jiangsu Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments, Southeast University, 211189, Nanjing, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Biomedical Microdevices |d Springer US; http://www.springer-ny.com |g 17/6(2015-12-01), 1-11 |x 1387-2176 |q 17:6<1 |1 2015 |2 17 |o 10544 | ||