caa a22 4500 605479615 CHVBK 20210128100411.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s10544-015-0018-y doi (NATIONALLICENCE)springer-10.1007/s10544-015-0018-y High-throughput blood cell focusing and plasma isolation using spiral inertial microfluidic devices [Elektronische Daten] [Nan Xiang, Zhonghua Ni] Herein, we explored the blood cell focusing and plasma isolation using a spiral inertial microfluidic device. First, the flow-rate and concentration effects on the migration dynamics of blood cells were systematically investigated to uncover the focusing mechanisms and steric crowding effects of cells in Dean-coupled inertial flows. A novel phenomenon that the focusing status of discoid red blood cells (RBCs) changes according to the channel height was discovered. These experimental data may provide valuable insights for the high-throughput processing of blood samples using inertial microfluidics. On the basis of the improved understandings on blood cell focusing, efficient isolation of plasma from whole blood with a 20-fold dilution was achieved at a throughput up to 700μl/min. The purity of the isolated blood plasma was close to 100%, and the plasma yield was calculated to be 38.5%. As compared with previously-reported devices, our spiral inertial microfluidic device provides a balanced overall performance, and has overriding advantages in terms of processing throughput and operating efficiency. Springer Science+Business Media New York, 2015 Blood cell focusing nationallicence Plasma isolation nationallicence Inertial microfluidics nationallicence High-throughput processing nationallicence Migration dynamics nationallicence Xiang Nan School of Mechanical Engineering, and Jiangsu Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments, Southeast University, 211189, Nanjing, China aut Ni Zhonghua School of Mechanical Engineering, and Jiangsu Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments, Southeast University, 211189, Nanjing, China aut Biomedical Microdevices Springer US; http://www.springer-ny.com 17/6(2015-12-01), 1-11 1387-2176 17:6<1 2015 17 10544 https://doi.org/10.1007/s10544-015-0018-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10544-015-0018-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Xiang Nan School of Mechanical Engineering, and Jiangsu Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments, Southeast University, 211189, Nanjing, China aut NATIONALLICENCE 700 1- Ni Zhonghua School of Mechanical Engineering, and Jiangsu Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments, Southeast University, 211189, Nanjing, China aut NATIONALLICENCE 773 0- Biomedical Microdevices Springer US; http://www.springer-ny.com 17/6(2015-12-01), 1-11 1387-2176 17:6<1 2015 17 10544