caa a22 4500 60547964X CHVBK 20210128100411.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s10544-015-0020-4 doi (NATIONALLICENCE)springer-10.1007/s10544-015-0020-4 Extended-gate field-effect transistor packed in micro channel for glucose, urea and protein biomarker detection [Elektronische Daten] [Yen-Heng Lin, Chih-Pin Chu, Chen-Fu Lin, Hsin-Hao Liao, Hann-Huei Tsai, Ying-Zong Juang] This study developed a packaging method to integrate the extended-gate field-effect transistor (EGFET) into a microfluidic chip as a biological sensor. In addition, we present two immobilization approaches for the bio-recognition that are appropriate to this chip, allowing it to measure the concentrations of hydrogen ions, glucose, urea, and specific proteins in a solution. Alginate-calcium microcubes were used to embed the enzymes and magnetic powder (enzyme carrier). When the sensing chip needs the enzyme for the catalytic reaction, the alginate microcubes containing the corresponding enzymes enter through the flow channel and are immobilized on the EGFET surface with an external magnet. High sensing performance of the chip is achieved, with 37.45mV/mM for measuring hydrogen ions at pH6-8 with a linearity of 0.9939, 7.00mV/mM for measuring glucose with a linearity of 0.9962, and 8.01mV/mM for measuring urea with a linearity of 0.9809. In addition, based on the principle of the immunoassay, the magnetic beads with the specific antibody were used to capture the target protein in the sample. Then, negatively charged DNA fragments bound to a secondary antibody were used to amplify the signal for EGFET measurement. The magnetic beads with completed immune response bonding were then fixed on the surface of the sensor by an external magnetic field. Therefore, the measured object can directly contact the sensor surface, and quantitative detection of the protein concentration can be achieved. Apolipoprotein A1 (APOA1) was detected as a target protein, with a minimum detection limit of approximately 12.5ng/mL. Springer Science+Business Media New York, 2015 Enzyme immobilization nationallicence Microfluidic nationallicence Extended-gate field-effect transistor nationallicence Protein nationallicence Lin Yen-Heng Department of Electronic Engineering, Chang Gung University, Taoyuan 333, Taiwan, Republic of China aut Chu Chih-Pin Department of Electronic Engineering, Chang Gung University, Taoyuan 333, Taiwan, Republic of China aut Lin Chen-Fu National Chip Implementation Center, Taiwan, Hsinchu, Republic of China aut Liao Hsin-Hao National Chip Implementation Center, Taiwan, Hsinchu, Republic of China aut Tsai Hann-Huei National Chip Implementation Center, Taiwan, Hsinchu, Republic of China aut Juang Ying-Zong National Chip Implementation Center, Taiwan, Hsinchu, Republic of China aut Biomedical Microdevices Springer US; http://www.springer-ny.com 17/6(2015-12-01), 1-9 1387-2176 17:6<1 2015 17 10544 https://doi.org/10.1007/s10544-015-0020-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10544-015-0020-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lin Yen-Heng Department of Electronic Engineering, Chang Gung University, Taoyuan 333, Taiwan, Republic of China aut NATIONALLICENCE 700 1- Chu Chih-Pin Department of Electronic Engineering, Chang Gung University, Taoyuan 333, Taiwan, Republic of China aut NATIONALLICENCE 700 1- Lin Chen-Fu National Chip Implementation Center, Taiwan, Hsinchu, Republic of China aut NATIONALLICENCE 700 1- Liao Hsin-Hao National Chip Implementation Center, Taiwan, Hsinchu, Republic of China aut NATIONALLICENCE 700 1- Tsai Hann-Huei National Chip Implementation Center, Taiwan, Hsinchu, Republic of China aut NATIONALLICENCE 700 1- Juang Ying-Zong National Chip Implementation Center, Taiwan, Hsinchu, Republic of China aut NATIONALLICENCE 773 0- Biomedical Microdevices Springer US; http://www.springer-ny.com 17/6(2015-12-01), 1-9 1387-2176 17:6<1 2015 17 10544