caa a22 4500 605479666 CHVBK 20210128100411.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s10544-015-9965-6 doi (NATIONALLICENCE)springer-10.1007/s10544-015-9965-6 Separation and assisted patterning of hippocampal neurons from glial cells using positive dielectrophoresis [Elektronische Daten] [Tianyi Zhou, Susan Perry, Yixuan Ming, Susanne Petryna, Vicki Fluck, Svetlana Tatic-Lucic] In this work, we describe the separation of embryonic mouse hippocampal neurons from glial cells using a positive dielectrophoresis (DEP) process. Here, we have implemented a cell trapping-favorable, cell suspension solution with low conductivity. It enables positive dielectrophoresis for hippocampal neurons (thereby attracting them to the electrodes), while resulting in negative dielectrophoresis for glial cells (repelling them from the electrodes). We have systematically performed a mathematical simulation and analysis to anticipate the DEP frequency at which hippocampal neurons and glial cells are separated. Simulated DEP crossover frequencies have been experimentally verified, and new, refined glial dielectric and physical properties are suggested that better reflect the experimental results obtained. DEP movements of neurons and glial cells in targeted separation media were experimentally analyzed, under the specified electric signal. Additionally, we have confirmed our modeling results by selectively trapping neurons over electrodes on a custom-made, multi-electrode array (MEA), resulting in active recruitment of neurons over the stimulation and recording sites. This technique is a valuable addition to the toolbox for creating more functional and versatile multi-electrode arrays. Springer Science+Business Media New York, 2015 Dielectrophoresis (DEP) nationallicence Separation nationallicence Patterning nationallicence Hippocampal neurons nationallicence Glial cells nationallicence Zhou Tianyi Department of Electrical and Computer Engineering, Lehigh University, 18015, Bethlehem, PA, USA aut Perry Susan Bioengineering Program, Lehigh University, 18015, Bethlehem, PA, USA aut Ming Yixuan Department of Electrical and Computer Engineering, Lehigh University, 18015, Bethlehem, PA, USA aut Petryna Susanne Bioengineering Program, Lehigh University, 18015, Bethlehem, PA, USA aut Fluck Vicki Bioengineering Program, Lehigh University, 18015, Bethlehem, PA, USA aut Tatic-Lucic Svetlana Department of Electrical and Computer Engineering, Lehigh University, 18015, Bethlehem, PA, USA aut Biomedical Microdevices Springer US; http://www.springer-ny.com 17/3(2015-06-01), 1-14 1387-2176 17:3<1 2015 17 10544 https://doi.org/10.1007/s10544-015-9965-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10544-015-9965-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhou Tianyi Department of Electrical and Computer Engineering, Lehigh University, 18015, Bethlehem, PA, USA aut NATIONALLICENCE 700 1- Perry Susan Bioengineering Program, Lehigh University, 18015, Bethlehem, PA, USA aut NATIONALLICENCE 700 1- Ming Yixuan Department of Electrical and Computer Engineering, Lehigh University, 18015, Bethlehem, PA, USA aut NATIONALLICENCE 700 1- Petryna Susanne Bioengineering Program, Lehigh University, 18015, Bethlehem, PA, USA aut NATIONALLICENCE 700 1- Fluck Vicki Bioengineering Program, Lehigh University, 18015, Bethlehem, PA, USA aut NATIONALLICENCE 700 1- Tatic-Lucic Svetlana Department of Electrical and Computer Engineering, Lehigh University, 18015, Bethlehem, PA, USA aut NATIONALLICENCE 773 0- Biomedical Microdevices Springer US; http://www.springer-ny.com 17/3(2015-06-01), 1-14 1387-2176 17:3<1 2015 17 10544