caa a22 4500 605479690 CHVBK 20210128100411.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s10544-015-9970-9 doi (NATIONALLICENCE)springer-10.1007/s10544-015-9970-9 A nanoliter self-priming compartmentalization chip for point-of-care digital PCR analysis [Elektronische Daten] [Qi Song, Yibo Gao, Qiangyuan Zhu, Qingchang Tian, Bingwen Yu, Bofan Song, Yanan Xu, Maokai Yuan, Congcong Ma, Wei Jin, Tao Zhang, Ying Mu, Qinhan Jin] A nanoliter self-priming compartmentalization (SPC) microfluidic chip suited for the digital polymerase chain reaction (dPCR) analysis in point-of-care testing (POCT) has been developed. This dPCR chip is fabricated of polydimethylsiloxane (PDMS). After the dPCR chip is evacuated, there will be a negative pressure environment in the chip because of the gas solubility of PDMS. The negative pressure environment can provide a self-priming power so that the sample solutions can be sucked into each reaction chamber sequentially. The whole sampling process requires no external power and is valve-free. Channels that contain water are designed around each sample panel to prevent the solvent (water) from evaporating during dPCR process. A glass coverslip is also used as a waterproof layer, which is more convenient and more efficient than other waterproof methods seen in literature. This dPCR chip allows three samples to be amplified at the same time. Each sample is distributed into 1040 reaction chambers, and each chamber is only 2.08nL. Human β-actin DNA solutions of known concentrations are used as the templates for the dPCR analyses to verify the sensitivity and accuracy of the method. Template DNA solutions diluted to concentrations of 300, 100 and 10 copies/μL are tested and shown that this simple, portable and self-priming dPCR chip can be used at any clinic as a real POCT technique. Springer Science+Business Media New York, 2015 Microfluidic chip nationallicence Digital PCR nationallicence Self-priming compartmentalization nationallicence POCT nationallicence Song Qi Research Center for Analytical Instrumentation, Institute of Cyber-Systems and Control, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou, People's Republic of China aut Gao Yibo Research Center for Analytical Instrumentation, Institute of Cyber-Systems and Control, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou, People's Republic of China aut Zhu Qiangyuan Research Center for Analytical Instrumentation, Institute of Cyber-Systems and Control, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou, People's Republic of China aut Tian Qingchang Research Center for Analytical Instrumentation, Institute of Cyber-Systems and Control, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou, People's Republic of China aut Yu Bingwen Research Center for Analytical Instrumentation, Institute of Cyber-Systems and Control, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou, People's Republic of China aut Song Bofan Research Center for Analytical Instrumentation, Institute of Cyber-Systems and Control, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou, People's Republic of China aut Xu Yanan Research Center for Analytical Instrumentation, Institute of Cyber-Systems and Control, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou, People's Republic of China aut Yuan Maokai Research Center for Analytical Instrumentation, Institute of Cyber-Systems and Control, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou, People's Republic of China aut Ma Congcong Research Center for Analytical Instrumentation, Institute of Cyber-Systems and Control, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou, People's Republic of China aut Jin Wei Research Center for Analytical Instrumentation, Institute of Cyber-Systems and Control, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou, People's Republic of China aut Zhang Tao Research Center for Analytical Instrumentation, Institute of Cyber-Systems and Control, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou, People's Republic of China aut Mu Ying Research Center for Analytical Instrumentation, Institute of Cyber-Systems and Control, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou, People's Republic of China aut Jin Qinhan Research Center for Analytical Instrumentation, Institute of Cyber-Systems and Control, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou, People's Republic of China aut Biomedical Microdevices Springer US; http://www.springer-ny.com 17/3(2015-06-01), 1-8 1387-2176 17:3<1 2015 17 10544 https://doi.org/10.1007/s10544-015-9970-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10544-015-9970-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Song Qi Research Center for Analytical Instrumentation, Institute of Cyber-Systems and Control, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Gao Yibo Research Center for Analytical Instrumentation, Institute of Cyber-Systems and Control, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Zhu Qiangyuan Research Center for Analytical Instrumentation, Institute of Cyber-Systems and Control, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Tian Qingchang Research Center for Analytical Instrumentation, Institute of Cyber-Systems and Control, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Yu Bingwen Research Center for Analytical Instrumentation, Institute of Cyber-Systems and Control, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Song Bofan Research Center for Analytical Instrumentation, Institute of Cyber-Systems and Control, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Xu Yanan Research Center for Analytical Instrumentation, Institute of Cyber-Systems and Control, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Yuan Maokai Research Center for Analytical Instrumentation, Institute of Cyber-Systems and Control, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Ma Congcong Research Center for Analytical Instrumentation, Institute of Cyber-Systems and Control, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Jin Wei Research Center for Analytical Instrumentation, Institute of Cyber-Systems and Control, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Tao Research Center for Analytical Instrumentation, Institute of Cyber-Systems and Control, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Mu Ying Research Center for Analytical Instrumentation, Institute of Cyber-Systems and Control, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Jin Qinhan Research Center for Analytical Instrumentation, Institute of Cyber-Systems and Control, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou, People's Republic of China aut NATIONALLICENCE 773 0- Biomedical Microdevices Springer US; http://www.springer-ny.com 17/3(2015-06-01), 1-8 1387-2176 17:3<1 2015 17 10544