caa a22 4500 605479704 CHVBK 20210128100411.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s10544-015-9955-8 doi (NATIONALLICENCE)springer-10.1007/s10544-015-9955-8 Simple microfluidic device for studying chemotaxis in response to dual gradients [Elektronische Daten] [S. Moussavi-Harami, H. Pezzi, A. Huttenlocher, D. Beebe] Chemotaxis is a fundamental biological process where complex chemotactic gradients are integrated and prioritized to guide cell migration toward specific locations. To understand the mechanisms of gradient dependent cell migration, it is important to develop in vitro models that recapitulate key attributes of the chemotactic cues present in vivo. Current in vitro tools for studying cell migration are not amenable to easily study the response of neutrophils to dual gradients. Many of these systems require external pumps and complex setups to establish and maintain the gradients. Here we report a simple yet innovative microfluidic device for studying cell migration in the presence of dual chemotactic gradients through a 3-dimensional substrate. The device is tested and validated by studying the migration of the neutrophil-like cell line PLB-985 to gradients of fMLP. Furthermore, the device is expanded and used with heparinised whole blood, whereupon neutrophils were observed to migrate from whole blood towards gradients of fMLP eliminating the need for any neutrophil purification or capture steps. Springer Science+Business Media New York, 2015 Migrations nationallicence Neutrophils nationallicence Chemotaxis nationallicence Gradients nationallicence Moussavi-Harami S. Department of Biomedical Engineering, University of Wisconsin, Madison, WI, USA aut Pezzi H. Department of Biomedical Engineering, University of Wisconsin, Madison, WI, USA aut Huttenlocher A. Department of Paediatrics, University of Wisconsin, Madison, WI, USA aut Beebe D. Department of Biomedical Engineering, University of Wisconsin, Madison, WI, USA aut Biomedical Microdevices Springer US; http://www.springer-ny.com 17/3(2015-06-01), 1-11 1387-2176 17:3<1 2015 17 10544 https://doi.org/10.1007/s10544-015-9955-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10544-015-9955-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Moussavi-Harami S. Department of Biomedical Engineering, University of Wisconsin, Madison, WI, USA aut NATIONALLICENCE 700 1- Pezzi H. Department of Biomedical Engineering, University of Wisconsin, Madison, WI, USA aut NATIONALLICENCE 700 1- Huttenlocher A. Department of Paediatrics, University of Wisconsin, Madison, WI, USA aut NATIONALLICENCE 700 1- Beebe D. Department of Biomedical Engineering, University of Wisconsin, Madison, WI, USA aut NATIONALLICENCE 773 0- Biomedical Microdevices Springer US; http://www.springer-ny.com 17/3(2015-06-01), 1-11 1387-2176 17:3<1 2015 17 10544