caa a22 4500 605479720 CHVBK 20210128100411.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s10544-015-9954-9 doi (NATIONALLICENCE)springer-10.1007/s10544-015-9954-9 Detection of an amphiphilic biosample in a paper microchannel based on length [Elektronische Daten] [Yu-Tzu Chen, Jing-Tang Yang] We developed a simple method to achieve semiquantitative detection of an amphiphilic biosample through measuring the length of flow on a microfluidic analytical device (μPAD) based on paper. When an amphiphilic sample was dripped into a straight microchannel defined with a printed wax barrier (hydrophobic) on filter paper (hydrophilic), the length of flow was affected by the reciprocal effect between the sample, the filter-paper channel and the wax barrier. The flow length decreased with increasing concentration of an amphiphilic sample because of adsorption of the sample on the hydrophobic barrier. Measurement of the flow length enabled a determination of the concentration of the amphiphilic sample. The several tested samples included surfactants (Tween 20 and Triton X-100), oligonucleotides (DNA), bovine serum albumin (BSA), human albumin, nitrite, glucose and low-density lipoprotein (LDL). The results show that the measurement of the flow length determined directly the concentration of an amphiphilic sample, whereas a non-amphiphilic sample was not amenable to this method. The proposed method features the advantages of small cost, simplicity, convenience, directness, rapidity (<5min) and requirement of only a small volume (5μL) of sample, with prospective applications in developing areas and sites near patients for testing at a point of care (POCT). Springer Science+Business Media New York, 2015 Amphiphilic nationallicence Protein nationallicence Length-based measurement nationallicence Semiquantitative analysis nationallicence Microfluidic paper-based analytical device (μPAD) nationallicence Chen Yu-Tzu Department of Mechanical Engineering, National Taiwan University, No.1, Sec.4, Roosevelt Road, 106, Taipei, Taiwan aut Yang Jing-Tang Department of Mechanical Engineering, National Taiwan University, No.1, Sec.4, Roosevelt Road, 106, Taipei, Taiwan aut Biomedical Microdevices Springer US; http://www.springer-ny.com 17/3(2015-06-01), 1-8 1387-2176 17:3<1 2015 17 10544 https://doi.org/10.1007/s10544-015-9954-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10544-015-9954-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chen Yu-Tzu Department of Mechanical Engineering, National Taiwan University, No.1, Sec.4, Roosevelt Road, 106, Taipei, Taiwan aut NATIONALLICENCE 700 1- Yang Jing-Tang Department of Mechanical Engineering, National Taiwan University, No.1, Sec.4, Roosevelt Road, 106, Taipei, Taiwan aut NATIONALLICENCE 773 0- Biomedical Microdevices Springer US; http://www.springer-ny.com 17/3(2015-06-01), 1-8 1387-2176 17:3<1 2015 17 10544