Microfluidics and numerical simulation as methods for standardization of zebrafish sperm cell activation
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| 024 | 7 | 0 | |a 10.1007/s10544-015-9957-6 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s10544-015-9957-6 | ||
| 245 | 0 | 0 | |a Microfluidics and numerical simulation as methods for standardization of zebrafish sperm cell activation |h [Elektronische Daten] |c [Thomas Scherr, Gerald Knapp, Amy Guitreau, Daniel Park, Terrence Tiersch, Krishnaswamy Nandakumar, W. Monroe] |
| 520 | 3 | |a Sperm cell activation plays a critical role in a range of biological and engineering processes, from fertilization to cryopreservation protocol evaluation. Across a range of species, ionic and osmotic effects have been discovered that lead to activation. Sperm cells of zebrafish (Danio rerio) initiate motility in a hypoosmotic environment. In this study, we employ a microfluidic mixer for the purpose of rapidly diluting the extracellular medium to initiate the onset of cell motility. The use of a microchannel offers a rapid and reproducible mixing profile throughout the device. This greatly reduces variability from trial to trial relative to the current methods of analysis. Coupling these experiments with numerical simulations, we were able to investigate the dynamics of intracellular osmolality as each cell moves along its path through the micromixer. Our results suggest that intracellular osmolality, and hence intracellular ion concentration, only slightly decreases, contrary to the common thought that larger changes in these parameters are required for activation. Utilizing this framework, microfluidics for controlled extracellular environments and associated numerical modeling, has practical applicability in standardizing high-throughput aquatic sperm activation, and more fundamentally, investigations of the intracellular environment leading to motility. | |
| 540 | |a Springer Science+Business Media New York, 2015 | ||
| 690 | 7 | |a Microfluidic |2 nationallicence | |
| 690 | 7 | |a Sperm analysis |2 nationallicence | |
| 690 | 7 | |a Numerical modeling |2 nationallicence | |
| 690 | 7 | |a Transport modeling |2 nationallicence | |
| 690 | 7 | |a Zebrafish sperm cell activation |2 nationallicence | |
| 700 | 1 | |a Scherr |D Thomas |u Department of Biomedical Engineering, Vanderbilt University, 37235, Nashville, TN, USA |4 aut | |
| 700 | 1 | |a Knapp |D Gerald |u Department of Mechanical and Industrial Engineering, Louisiana State University, 70803, Baton Rouge, LA, USA |4 aut | |
| 700 | 1 | |a Guitreau |D Amy |u Aquatic Germplasm and Genetic Resources Center, LSU Agricultural Center, 70803, Baton Rouge, LA, USA |4 aut | |
| 700 | 1 | |a Park |D Daniel |u Department of Mechanical and Industrial Engineering, Louisiana State University, 70803, Baton Rouge, LA, USA |4 aut | |
| 700 | 1 | |a Tiersch |D Terrence |u Aquatic Germplasm and Genetic Resources Center, LSU Agricultural Center, 70803, Baton Rouge, LA, USA |4 aut | |
| 700 | 1 | |a Nandakumar |D Krishnaswamy |u Cain Department of Chemical Engineering, Louisiana State University, 70803, Baton Rouge, LA, USA |4 aut | |
| 700 | 1 | |a Monroe |D W. |u Department of Biological and Agricultural Engineering, Louisiana State University and LSU Agricultural Center, 163 EB Doran, 70803, Baton Rouge, LA, USA |4 aut | |
| 773 | 0 | |t Biomedical Microdevices |d Springer US; http://www.springer-ny.com |g 17/3(2015-06-01), 1-10 |x 1387-2176 |q 17:3<1 |1 2015 |2 17 |o 10544 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s10544-015-9957-6 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s10544-015-9957-6 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Scherr |D Thomas |u Department of Biomedical Engineering, Vanderbilt University, 37235, Nashville, TN, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Knapp |D Gerald |u Department of Mechanical and Industrial Engineering, Louisiana State University, 70803, Baton Rouge, LA, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Guitreau |D Amy |u Aquatic Germplasm and Genetic Resources Center, LSU Agricultural Center, 70803, Baton Rouge, LA, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Park |D Daniel |u Department of Mechanical and Industrial Engineering, Louisiana State University, 70803, Baton Rouge, LA, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Tiersch |D Terrence |u Aquatic Germplasm and Genetic Resources Center, LSU Agricultural Center, 70803, Baton Rouge, LA, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Nandakumar |D Krishnaswamy |u Cain Department of Chemical Engineering, Louisiana State University, 70803, Baton Rouge, LA, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Monroe |D W. |u Department of Biological and Agricultural Engineering, Louisiana State University and LSU Agricultural Center, 163 EB Doran, 70803, Baton Rouge, LA, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Biomedical Microdevices |d Springer US; http://www.springer-ny.com |g 17/3(2015-06-01), 1-10 |x 1387-2176 |q 17:3<1 |1 2015 |2 17 |o 10544 | ||