caa a22 4500 605479755 CHVBK 20210128100411.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s10544-015-9957-6 doi (NATIONALLICENCE)springer-10.1007/s10544-015-9957-6 Microfluidics and numerical simulation as methods for standardization of zebrafish sperm cell activation [Elektronische Daten] [Thomas Scherr, Gerald Knapp, Amy Guitreau, Daniel Park, Terrence Tiersch, Krishnaswamy Nandakumar, W. Monroe] Sperm cell activation plays a critical role in a range of biological and engineering processes, from fertilization to cryopreservation protocol evaluation. Across a range of species, ionic and osmotic effects have been discovered that lead to activation. Sperm cells of zebrafish (Danio rerio) initiate motility in a hypoosmotic environment. In this study, we employ a microfluidic mixer for the purpose of rapidly diluting the extracellular medium to initiate the onset of cell motility. The use of a microchannel offers a rapid and reproducible mixing profile throughout the device. This greatly reduces variability from trial to trial relative to the current methods of analysis. Coupling these experiments with numerical simulations, we were able to investigate the dynamics of intracellular osmolality as each cell moves along its path through the micromixer. Our results suggest that intracellular osmolality, and hence intracellular ion concentration, only slightly decreases, contrary to the common thought that larger changes in these parameters are required for activation. Utilizing this framework, microfluidics for controlled extracellular environments and associated numerical modeling, has practical applicability in standardizing high-throughput aquatic sperm activation, and more fundamentally, investigations of the intracellular environment leading to motility. Springer Science+Business Media New York, 2015 Microfluidic nationallicence Sperm analysis nationallicence Numerical modeling nationallicence Transport modeling nationallicence Zebrafish sperm cell activation nationallicence Scherr Thomas Department of Biomedical Engineering, Vanderbilt University, 37235, Nashville, TN, USA aut Knapp Gerald Department of Mechanical and Industrial Engineering, Louisiana State University, 70803, Baton Rouge, LA, USA aut Guitreau Amy Aquatic Germplasm and Genetic Resources Center, LSU Agricultural Center, 70803, Baton Rouge, LA, USA aut Park Daniel Department of Mechanical and Industrial Engineering, Louisiana State University, 70803, Baton Rouge, LA, USA aut Tiersch Terrence Aquatic Germplasm and Genetic Resources Center, LSU Agricultural Center, 70803, Baton Rouge, LA, USA aut Nandakumar Krishnaswamy Cain Department of Chemical Engineering, Louisiana State University, 70803, Baton Rouge, LA, USA aut Monroe W. Department of Biological and Agricultural Engineering, Louisiana State University and LSU Agricultural Center, 163 EB Doran, 70803, Baton Rouge, LA, USA aut Biomedical Microdevices Springer US; http://www.springer-ny.com 17/3(2015-06-01), 1-10 1387-2176 17:3<1 2015 17 10544 https://doi.org/10.1007/s10544-015-9957-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10544-015-9957-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Scherr Thomas Department of Biomedical Engineering, Vanderbilt University, 37235, Nashville, TN, USA aut NATIONALLICENCE 700 1- Knapp Gerald Department of Mechanical and Industrial Engineering, Louisiana State University, 70803, Baton Rouge, LA, USA aut NATIONALLICENCE 700 1- Guitreau Amy Aquatic Germplasm and Genetic Resources Center, LSU Agricultural Center, 70803, Baton Rouge, LA, USA aut NATIONALLICENCE 700 1- Park Daniel Department of Mechanical and Industrial Engineering, Louisiana State University, 70803, Baton Rouge, LA, USA aut NATIONALLICENCE 700 1- Tiersch Terrence Aquatic Germplasm and Genetic Resources Center, LSU Agricultural Center, 70803, Baton Rouge, LA, USA aut NATIONALLICENCE 700 1- Nandakumar Krishnaswamy Cain Department of Chemical Engineering, Louisiana State University, 70803, Baton Rouge, LA, USA aut NATIONALLICENCE 700 1- Monroe W. Department of Biological and Agricultural Engineering, Louisiana State University and LSU Agricultural Center, 163 EB Doran, 70803, Baton Rouge, LA, USA aut NATIONALLICENCE 773 0- Biomedical Microdevices Springer US; http://www.springer-ny.com 17/3(2015-06-01), 1-10 1387-2176 17:3<1 2015 17 10544