A microfluidic cell culture device (μFCCD) to culture epithelial cells with physiological and morphological properties that mimic those of the human intestine
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| 024 | 7 | 0 | |a 10.1007/s10544-015-9966-5 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s10544-015-9966-5 | ||
| 245 | 0 | 2 | |a A microfluidic cell culture device (μFCCD) to culture epithelial cells with physiological and morphological properties that mimic those of the human intestine |h [Elektronische Daten] |c [Meiying Chi, Banya Yi, Seunghan Oh, Dong-June Park, Jong Sung, Sungsu Park] |
| 520 | 3 | |a Physiological and morphological properties of the human intestine cannot be accurately mimicked in conventional culture devices such as well plates and petri dishes where intestinal epithelial cells form a monolayer with loose contacts among cells. Here, we report a novel microfluidic cell culture device (μFCCD) that can be used to culture cells as a human intestinal model. This device enables intestinal epithelial cells (Caco-2) to grow three-dimensionally on a porous membrane coated with fibronectin between two polydimethylsiloxane (PDMS) layers. Within 3days, Caco-2 cells cultured in the μFCCD formed villi- and crypt-like structures with small intercellular spaces, while individual cells were tightly connected to one another through the expression of the tight junction protein occludin, and were covered with a secreted mucin, MUC-2. Caco-2 cells cultured in the μFCCD for 3days were less susceptible to bacterial attack than those cultured in transwell plates for 21days. μFCCD-cultured Caco-2 cells also displayed physiologically relevant absorption and paracellular transport properties. These results suggest that our intestinal model more accurately mimics the morphological and physiological properties of the intestine in vivo than the conventional transwell culture model. | |
| 540 | |a Springer Science+Business Media New York, 2015 | ||
| 690 | 7 | |a Caco-2 |2 nationallicence | |
| 690 | 7 | |a μFCCD |2 nationallicence | |
| 690 | 7 | |a Intestine |2 nationallicence | |
| 690 | 7 | |a Mucin-2 |2 nationallicence | |
| 690 | 7 | |a Microorganisms |2 nationallicence | |
| 700 | 1 | |a Chi |D Meiying |u Department of Chemistry and Nano Sciences (BK21 plus), EwhaWomans University, 120-750, Seoul, Korea |4 aut | |
| 700 | 1 | |a Yi |D Banya |u Department of Chemical Engineering, Hongik University, 121-791, Seoul, Korea |4 aut | |
| 700 | 1 | |a Oh |D Seunghan |u Department of Chemistry and Nano Sciences (BK21 plus), EwhaWomans University, 120-750, Seoul, Korea |4 aut | |
| 700 | 1 | |a Park |D Dong-June |u Korea Food Research Institute, 463-746, Seongnam, Korea |4 aut | |
| 700 | 1 | |a Sung |D Jong |u Department of Chemical Engineering, Hongik University, 121-791, Seoul, Korea |4 aut | |
| 700 | 1 | |a Park |D Sungsu |u School of Mechanical Engineering, Sungkyunkwan University, 440-746, Suwon, Korea |4 aut | |
| 773 | 0 | |t Biomedical Microdevices |d Springer US; http://www.springer-ny.com |g 17/3(2015-06-01), 1-10 |x 1387-2176 |q 17:3<1 |1 2015 |2 17 |o 10544 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s10544-015-9966-5 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s10544-015-9966-5 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Chi |D Meiying |u Department of Chemistry and Nano Sciences (BK21 plus), EwhaWomans University, 120-750, Seoul, Korea |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Yi |D Banya |u Department of Chemical Engineering, Hongik University, 121-791, Seoul, Korea |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Oh |D Seunghan |u Department of Chemistry and Nano Sciences (BK21 plus), EwhaWomans University, 120-750, Seoul, Korea |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Park |D Dong-June |u Korea Food Research Institute, 463-746, Seongnam, Korea |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Sung |D Jong |u Department of Chemical Engineering, Hongik University, 121-791, Seoul, Korea |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Park |D Sungsu |u School of Mechanical Engineering, Sungkyunkwan University, 440-746, Suwon, Korea |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Biomedical Microdevices |d Springer US; http://www.springer-ny.com |g 17/3(2015-06-01), 1-10 |x 1387-2176 |q 17:3<1 |1 2015 |2 17 |o 10544 | ||