caa a22 4500 60547978X CHVBK 20210128100411.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s10544-015-9966-5 doi (NATIONALLICENCE)springer-10.1007/s10544-015-9966-5 A microfluidic cell culture device (μFCCD) to culture epithelial cells with physiological and morphological properties that mimic those of the human intestine [Elektronische Daten] [Meiying Chi, Banya Yi, Seunghan Oh, Dong-June Park, Jong Sung, Sungsu Park] Physiological and morphological properties of the human intestine cannot be accurately mimicked in conventional culture devices such as well plates and petri dishes where intestinal epithelial cells form a monolayer with loose contacts among cells. Here, we report a novel microfluidic cell culture device (μFCCD) that can be used to culture cells as a human intestinal model. This device enables intestinal epithelial cells (Caco-2) to grow three-dimensionally on a porous membrane coated with fibronectin between two polydimethylsiloxane (PDMS) layers. Within 3days, Caco-2 cells cultured in the μFCCD formed villi- and crypt-like structures with small intercellular spaces, while individual cells were tightly connected to one another through the expression of the tight junction protein occludin, and were covered with a secreted mucin, MUC-2. Caco-2 cells cultured in the μFCCD for 3days were less susceptible to bacterial attack than those cultured in transwell plates for 21days. μFCCD-cultured Caco-2 cells also displayed physiologically relevant absorption and paracellular transport properties. These results suggest that our intestinal model more accurately mimics the morphological and physiological properties of the intestine in vivo than the conventional transwell culture model. Springer Science+Business Media New York, 2015 Caco-2 nationallicence μFCCD nationallicence Intestine nationallicence Mucin-2 nationallicence Microorganisms nationallicence Chi Meiying Department of Chemistry and Nano Sciences (BK21 plus), EwhaWomans University, 120-750, Seoul, Korea aut Yi Banya Department of Chemical Engineering, Hongik University, 121-791, Seoul, Korea aut Oh Seunghan Department of Chemistry and Nano Sciences (BK21 plus), EwhaWomans University, 120-750, Seoul, Korea aut Park Dong-June Korea Food Research Institute, 463-746, Seongnam, Korea aut Sung Jong Department of Chemical Engineering, Hongik University, 121-791, Seoul, Korea aut Park Sungsu School of Mechanical Engineering, Sungkyunkwan University, 440-746, Suwon, Korea aut Biomedical Microdevices Springer US; http://www.springer-ny.com 17/3(2015-06-01), 1-10 1387-2176 17:3<1 2015 17 10544 https://doi.org/10.1007/s10544-015-9966-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10544-015-9966-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chi Meiying Department of Chemistry and Nano Sciences (BK21 plus), EwhaWomans University, 120-750, Seoul, Korea aut NATIONALLICENCE 700 1- Yi Banya Department of Chemical Engineering, Hongik University, 121-791, Seoul, Korea aut NATIONALLICENCE 700 1- Oh Seunghan Department of Chemistry and Nano Sciences (BK21 plus), EwhaWomans University, 120-750, Seoul, Korea aut NATIONALLICENCE 700 1- Park Dong-June Korea Food Research Institute, 463-746, Seongnam, Korea aut NATIONALLICENCE 700 1- Sung Jong Department of Chemical Engineering, Hongik University, 121-791, Seoul, Korea aut NATIONALLICENCE 700 1- Park Sungsu School of Mechanical Engineering, Sungkyunkwan University, 440-746, Suwon, Korea aut NATIONALLICENCE 773 0- Biomedical Microdevices Springer US; http://www.springer-ny.com 17/3(2015-06-01), 1-10 1387-2176 17:3<1 2015 17 10544