caa a22 4500 605479798 CHVBK 20210128100411.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s10544-015-9964-7 doi (NATIONALLICENCE)springer-10.1007/s10544-015-9964-7 Enrichment of circulating tumor cells in tumor-bearing mouse blood by a deterministic lateral displacement microfluidic device [Elektronische Daten] [Hiromasa Okano, Tomoki Konishi, Toshihiro Suzuki, Takahiro Suzuki, Shinya Ariyasu, Shin Aoki, Ryo Abe, Masanori Hayase] Concentration of real tumor cells leaking into blood from cancer was attempted by a deterministic lateral displacement (DLD) microfluidic device. Spiked cultured cell line tumor cells are often used to verify performance of the circulating tumor cells (CTCs) separation methods. Cultured tumor cells are obviously larger than most of hematocytes and considered not to be appropriate as CTC mimics, while there is uncertainty in identifying real CTCs from clinical samples and there is no practical way to examine CTCs leakage into benign cells during the sorting. In this work, blood samples were prepared from tumor-bearing mice whose tumors were induced by implanting cells with GFP expression to living mice. Therefore, CTCs were identified by their fluorescence emission. We succeeded in the enrichment of tumor cells to 0.05% from the blood, in which CTCs were negligibly detected among three million blood cells, and little loss of CTCs was observed. Springer Science+Business Media New York, 2015 Circulating tumor cell nationallicence Deterministic lateral displacement nationallicence Microfluidics nationallicence Tumor-bearing mouse nationallicence Cell sorting nationallicence Okano Hiromasa Department of Mechanical Engineering, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, 278-8510, Noda-shi, Chiba-ken, Japan aut Konishi Tomoki Department of Mechanical Engineering, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, 278-8510, Noda-shi, Chiba-ken, Japan aut Suzuki Toshihiro Center for Technologies Against Cancer, Research Institute of Science and Technology, Tokyo University of Science, 2669 Yamazaki, 278-0022, Noda-shi, Chiba-ken, Japan aut Suzuki Takahiro Department of Mechanical Engineering, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, 278-8510, Noda-shi, Chiba-ken, Japan aut Ariyasu Shinya Center for Technologies Against Cancer, Research Institute of Science and Technology, Tokyo University of Science, 2669 Yamazaki, 278-0022, Noda-shi, Chiba-ken, Japan aut Aoki Shin Center for Technologies Against Cancer, Research Institute of Science and Technology, Tokyo University of Science, 2669 Yamazaki, 278-0022, Noda-shi, Chiba-ken, Japan aut Abe Ryo Center for Technologies Against Cancer, Research Institute of Science and Technology, Tokyo University of Science, 2669 Yamazaki, 278-0022, Noda-shi, Chiba-ken, Japan aut Hayase Masanori Department of Mechanical Engineering, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, 278-8510, Noda-shi, Chiba-ken, Japan aut Biomedical Microdevices Springer US; http://www.springer-ny.com 17/3(2015-06-01), 1-11 1387-2176 17:3<1 2015 17 10544 https://doi.org/10.1007/s10544-015-9964-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10544-015-9964-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Okano Hiromasa Department of Mechanical Engineering, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, 278-8510, Noda-shi, Chiba-ken, Japan aut NATIONALLICENCE 700 1- Konishi Tomoki Department of Mechanical Engineering, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, 278-8510, Noda-shi, Chiba-ken, Japan aut NATIONALLICENCE 700 1- Suzuki Toshihiro Center for Technologies Against Cancer, Research Institute of Science and Technology, Tokyo University of Science, 2669 Yamazaki, 278-0022, Noda-shi, Chiba-ken, Japan aut NATIONALLICENCE 700 1- Suzuki Takahiro Department of Mechanical Engineering, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, 278-8510, Noda-shi, Chiba-ken, Japan aut NATIONALLICENCE 700 1- Ariyasu Shinya Center for Technologies Against Cancer, Research Institute of Science and Technology, Tokyo University of Science, 2669 Yamazaki, 278-0022, Noda-shi, Chiba-ken, Japan aut NATIONALLICENCE 700 1- Aoki Shin Center for Technologies Against Cancer, Research Institute of Science and Technology, Tokyo University of Science, 2669 Yamazaki, 278-0022, Noda-shi, Chiba-ken, Japan aut NATIONALLICENCE 700 1- Abe Ryo Center for Technologies Against Cancer, Research Institute of Science and Technology, Tokyo University of Science, 2669 Yamazaki, 278-0022, Noda-shi, Chiba-ken, Japan aut NATIONALLICENCE 700 1- Hayase Masanori Department of Mechanical Engineering, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, 278-8510, Noda-shi, Chiba-ken, Japan aut NATIONALLICENCE 773 0- Biomedical Microdevices Springer US; http://www.springer-ny.com 17/3(2015-06-01), 1-11 1387-2176 17:3<1 2015 17 10544