Engineered self-assembling monolayers for label free detection of influenza nucleoprotein
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| 024 | 7 | 0 | |a 10.1007/s10544-015-9951-z |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s10544-015-9951-z | ||
| 245 | 0 | 0 | |a Engineered self-assembling monolayers for label free detection of influenza nucleoprotein |h [Elektronische Daten] |c [Anton Le Brun, Andrei Soliakov, Deepan Shah, Stephen Holt, Alison McGill, Jeremy Lakey] |
| 520 | 3 | |a Integrating nanotechnology into useable devices requires a combination of bottom up and top down methodology. Often the techniques to measure and control these different components are entirely different, so methods that can analyse the nanoscale component in situ are of increasing importance. Here we describe a strategy that employs a self-assembling monolayer of engineered protein chimeras to display an array of oriented antibodies (IgG) on a microelectronic device for the label free detection of influenza nucleoprotein. The structural and functional properties of the bio-interface were characterised by a range of physical techniques including surface plasmon resonance, quartz-crystal microbalance and neutron reflectometry. This combination of methods reveals a 13.5nm thick engineered-monolayer that (i) self-assembles on gold surfaces, (ii) captures IgG with high affinity in a defined orientation and (iii) specifically recognises the influenza A nucleoprotein. Furthermore we also show that this non-covalent self-assembled structure can render the dissociation of bound IgG irreversible by chemical crosslinking in situ without affecting the IgG function. The methods can thus describe in detail the transition from soluble engineered molecules with nanometre dimensions to an array that demonstrates the principles of a working influenza sensor. | |
| 540 | |a The Author(s), 2015 | ||
| 690 | 7 | |a Protein engineering |2 nationallicence | |
| 690 | 7 | |a Surface plasmon resonance |2 nationallicence | |
| 690 | 7 | |a Quartz-crystal microbalance |2 nationallicence | |
| 690 | 7 | |a Neutron reflectometry |2 nationallicence | |
| 690 | 7 | |a Self-assembled monolayer |2 nationallicence | |
| 690 | 7 | |a Influenza |2 nationallicence | |
| 690 | 7 | |a BS(PEG)5 : Bis(succinimidyl) penta(ethylene glycol) |2 nationallicence | |
| 690 | 7 | |a BSSS : Bis(sulfosuccinimidyl) suberate |2 nationallicence | |
| 690 | 7 | |a ctOmpA : Circularly permuted transmembrane domain of E. coli outer membrane protein A |2 nationallicence | |
| 690 | 7 | |a GGZctOmpA : N-terminal tandem pair of B domains from protein G with a C-terminal ctOmpA scaffold |2 nationallicence | |
| 690 | 7 | |a IgG : Immunoglobulin G |2 nationallicence | |
| 690 | 7 | |a mAb245 : Mouse monoclonal IgG raised against rNP |2 nationallicence | |
| 690 | 7 | |a mAb108 : Mouse monoclonal IgG raised against rNP (different epitope to mAb245) |2 nationallicence | |
| 690 | 7 | |a NP : Native nucleoprotein from influenza |2 nationallicence | |
| 690 | 7 | |a NR : Neutron reflectometry |2 nationallicence | |
| 690 | 7 | |a nSLD : Neutron scattering length density |2 nationallicence | |
| 690 | 7 | |a PBS : Phosphate buffered saline |2 nationallicence | |
| 690 | 7 | |a QCM-D : Quartz-crystal microbalance with dissipation monitoring |2 nationallicence | |
| 690 | 7 | |a rNP : Recombinant nucleoprotein |2 nationallicence | |
| 690 | 7 | |a SPR : Surface plasmon resonance |2 nationallicence | |
| 690 | 7 | |a thioPEG : 1-mercaptoundec-11-yltriethylene glycol |2 nationallicence | |
| 700 | 1 | |a Le Brun |D Anton |u Bragg Institute, Australian Nuclear Science and Technology Organisation, Locked Bag 2001, 2232, Kirrawee DC, NSW, Australia |4 aut | |
| 700 | 1 | |a Soliakov |D Andrei |u Institute for Cell and Molecular Biosciences, The Medical School, Newcastle University, Framlington Place, NE2 4HH, Newcastle upon Tyne, UK |4 aut | |
| 700 | 1 | |a Shah |D Deepan |u Orla Protein Technologies Ltd, Biosciences Centre, International Centre for Life, Times Square, NE1 4EP, Newcastle upon Tyne, UK |4 aut | |
| 700 | 1 | |a Holt |D Stephen |u Bragg Institute, Australian Nuclear Science and Technology Organisation, Locked Bag 2001, 2232, Kirrawee DC, NSW, Australia |4 aut | |
| 700 | 1 | |a McGill |D Alison |u Institute for Cell and Molecular Biosciences, The Medical School, Newcastle University, Framlington Place, NE2 4HH, Newcastle upon Tyne, UK |4 aut | |
| 700 | 1 | |a Lakey |D Jeremy |u Institute for Cell and Molecular Biosciences, The Medical School, Newcastle University, Framlington Place, NE2 4HH, Newcastle upon Tyne, UK |4 aut | |
| 773 | 0 | |t Biomedical Microdevices |d Springer US; http://www.springer-ny.com |g 17/3(2015-06-01), 1-10 |x 1387-2176 |q 17:3<1 |1 2015 |2 17 |o 10544 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s10544-015-9951-z |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s10544-015-9951-z |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Le Brun |D Anton |u Bragg Institute, Australian Nuclear Science and Technology Organisation, Locked Bag 2001, 2232, Kirrawee DC, NSW, Australia |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Soliakov |D Andrei |u Institute for Cell and Molecular Biosciences, The Medical School, Newcastle University, Framlington Place, NE2 4HH, Newcastle upon Tyne, UK |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Shah |D Deepan |u Orla Protein Technologies Ltd, Biosciences Centre, International Centre for Life, Times Square, NE1 4EP, Newcastle upon Tyne, UK |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Holt |D Stephen |u Bragg Institute, Australian Nuclear Science and Technology Organisation, Locked Bag 2001, 2232, Kirrawee DC, NSW, Australia |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a McGill |D Alison |u Institute for Cell and Molecular Biosciences, The Medical School, Newcastle University, Framlington Place, NE2 4HH, Newcastle upon Tyne, UK |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Lakey |D Jeremy |u Institute for Cell and Molecular Biosciences, The Medical School, Newcastle University, Framlington Place, NE2 4HH, Newcastle upon Tyne, UK |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Biomedical Microdevices |d Springer US; http://www.springer-ny.com |g 17/3(2015-06-01), 1-10 |x 1387-2176 |q 17:3<1 |1 2015 |2 17 |o 10544 | ||