caa a22 4500 605479836 CHVBK 20210128100412.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s10544-015-9958-5 doi (NATIONALLICENCE)springer-10.1007/s10544-015-9958-5 pH-triggered drug release from biodegradable microwells for oral drug delivery [Elektronische Daten] [Line Nielsen, Johan Nagstrup, Sarah Gordon, Stephan Keller, Jesper Østergaard, Thomas Rades, Anette Müllertz, Anja Boisen] Microwells fabricated from poly-L-lactic acid (PLLA) were evaluated for their application as an oral drug delivery system using the amorphous sodium salt of furosemide (ASSF) as a model drug. Hot embossing of PLLA resulted in fabrication of microwells with an inner diameter of 240μm and a height of 100μm. The microwells were filled with ASSF using a modified screen printing technique, followed by coating of the microwell cavities with a gastro-resistant lid of Eudragit® L100. The release behavior of ASSF from the coated microwells was investigated using a μ-Diss profiler and a UV imaging system, and under conditions simulating the changing environment of the gastrointestinal tract. Biorelevant gastric medium (pH1.6) was employed, after which a change to biorelevant intestinal release medium (pH6.5) was carried out. Both μ-Diss profiler and UV imaging release experiments showed that sealing of microwell cavities with an Eudragit® layer prevented drug release in biorelevant gastric medium. An immediate release of the ASSF from coated microwells was observed in the intestinal medium. This pH-triggered release behavior demonstrates the future potential of PLLA microwells as a site-specific oral drug delivery system. Springer Science+Business Media New York, 2015 Biodegradable polymer nationallicence Oral drug delivery nationallicence Micro delivery systems nationallicence Furosemide nationallicence μ-Diss profiler nationallicence UV imaging nationallicence Nielsen Line Department of Micro- and Nanotechnology, Technical University of Denmark, Ørsteds Plads, Building 345B, Kongens Lyngby, Denmark aut Nagstrup Johan Department of Micro- and Nanotechnology, Technical University of Denmark, Ørsteds Plads, Building 345B, Kongens Lyngby, Denmark aut Gordon Sarah Department of Drug Delivery, Helmholtz Institute for Pharmaceutical Research Saarland (HIPS), Saarland University, Saarbrücken, Germany aut Keller Stephan Department of Micro- and Nanotechnology, Technical University of Denmark, Ørsteds Plads, Building 345B, Kongens Lyngby, Denmark aut Østergaard Jesper Department of Pharmacy, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark aut Rades Thomas Department of Pharmacy, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark aut Müllertz Anette Department of Pharmacy, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark aut Boisen Anja Department of Micro- and Nanotechnology, Technical University of Denmark, Ørsteds Plads, Building 345B, Kongens Lyngby, Denmark aut Biomedical Microdevices Springer US; http://www.springer-ny.com 17/3(2015-06-01), 1-7 1387-2176 17:3<1 2015 17 10544 https://doi.org/10.1007/s10544-015-9958-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10544-015-9958-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nielsen Line Department of Micro- and Nanotechnology, Technical University of Denmark, Ørsteds Plads, Building 345B, Kongens Lyngby, Denmark aut NATIONALLICENCE 700 1- Nagstrup Johan Department of Micro- and Nanotechnology, Technical University of Denmark, Ørsteds Plads, Building 345B, Kongens Lyngby, Denmark aut NATIONALLICENCE 700 1- Gordon Sarah Department of Drug Delivery, Helmholtz Institute for Pharmaceutical Research Saarland (HIPS), Saarland University, Saarbrücken, Germany aut NATIONALLICENCE 700 1- Keller Stephan Department of Micro- and Nanotechnology, Technical University of Denmark, Ørsteds Plads, Building 345B, Kongens Lyngby, Denmark aut NATIONALLICENCE 700 1- Østergaard Jesper Department of Pharmacy, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark aut NATIONALLICENCE 700 1- Rades Thomas Department of Pharmacy, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark aut NATIONALLICENCE 700 1- Müllertz Anette Department of Pharmacy, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark aut NATIONALLICENCE 700 1- Boisen Anja Department of Micro- and Nanotechnology, Technical University of Denmark, Ørsteds Plads, Building 345B, Kongens Lyngby, Denmark aut NATIONALLICENCE 773 0- Biomedical Microdevices Springer US; http://www.springer-ny.com 17/3(2015-06-01), 1-7 1387-2176 17:3<1 2015 17 10544