caa a22 4500 605479852 CHVBK 20210128100412.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s10544-015-9971-8 doi (NATIONALLICENCE)springer-10.1007/s10544-015-9971-8 Pump-free gradient-based micro-device enables quantitative and high-throughput bacterial growth inhibition analysis [Elektronische Daten] [Min Ran, Ying Wang, Sida Wang, Chunxiong Luo] Antibiotic susceptibility testing is very important in antibiotic therapy. Traditional methods to determine antibiotic susceptibility include disk diffusion and broth dilution. However, these tests are always labor intensive, time-consuming, and need large amounts of reagents. In this paper, we demonstrated a novel pump-free micro-device that enables quantitative and high-throughput bacterial growth inhibition analysis. This device consists of a series of wells and diffusion-based antibiotic gradient channels. The wells serve as antibiotic sources and buffer sinks, and we could easily observe the bacterial growth in the gradient channels .The design of the multi-wells is adapted to the commercialized multi-channel pipette, which makes it very convenient for loading reagents into the wells. For each assay, only about 20μL antibiotic solution is needed. As a demonstration, we used both fluorescence images and dark-field images to quantify the bacterial growth inhibition effect under different antibiotics. The quantitative data of bacterial growth inhibition under different antibiotics can be obtained within 3 to 4h. Considering the simple operation process and the high-throughput and quantitative result this device can offer, it has great potential to be widely used in clinics and may be useful for the study of the kinetics of bacterial growth. Springer Science+Business Media New York, 2015 High-throughput nationallicence Antibiotic susceptibility testing nationallicence Chemical gradient nationallicence Bacterial growth inhibition nationallicence Pump-free nationallicence Ran Min The State Key Laboratory for Artificial Microstructures and Mesoscopic Physics, School of Physics, Peking University, Beijing, China aut Wang Ying The State Key Laboratory for Artificial Microstructures and Mesoscopic Physics, School of Physics, Peking University, Beijing, China aut Wang Sida The State Key Laboratory for Artificial Microstructures and Mesoscopic Physics, School of Physics, Peking University, Beijing, China aut Luo Chunxiong The State Key Laboratory for Artificial Microstructures and Mesoscopic Physics, School of Physics, Peking University, Beijing, China aut Biomedical Microdevices Springer US; http://www.springer-ny.com 17/4(2015-08-01), 1-7 1387-2176 17:4<1 2015 17 10544 https://doi.org/10.1007/s10544-015-9971-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10544-015-9971-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ran Min The State Key Laboratory for Artificial Microstructures and Mesoscopic Physics, School of Physics, Peking University, Beijing, China aut NATIONALLICENCE 700 1- Wang Ying The State Key Laboratory for Artificial Microstructures and Mesoscopic Physics, School of Physics, Peking University, Beijing, China aut NATIONALLICENCE 700 1- Wang Sida The State Key Laboratory for Artificial Microstructures and Mesoscopic Physics, School of Physics, Peking University, Beijing, China aut NATIONALLICENCE 700 1- Luo Chunxiong The State Key Laboratory for Artificial Microstructures and Mesoscopic Physics, School of Physics, Peking University, Beijing, China aut NATIONALLICENCE 773 0- Biomedical Microdevices Springer US; http://www.springer-ny.com 17/4(2015-08-01), 1-7 1387-2176 17:4<1 2015 17 10544