caa a22 4500 605479895 CHVBK 20210128100412.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s10544-015-9990-5 doi (NATIONALLICENCE)springer-10.1007/s10544-015-9990-5 Rapid homogeneous endothelialization of high aspect ratio microvascular networks [Elektronische Daten] [Nisarga Naik, Donny Hanjaya-Putra, Carolyn Haller, Mark Allen, Elliot Chaikof] Microvascularization of an engineered tissue construct is necessary to ensure the nourishment and viability of the hosted cells. Microvascular constructs can be created by seeding the luminal surfaces of microfluidic channel arrays with endothelial cells. However, in a conventional flow-based system, the uniformity of endothelialization of such an engineered microvascular network is constrained by mass transfer of the cells through high length-to-diameter (L/D) aspect ratio microchannels. Moreover, given the inherent limitations of the initial seeding process to generate a uniform cell coating, the large surface-area-to-volume ratio of microfluidic systems demands long culture periods for the formation of confluent cellular microconduits. In this report, we describe the design of polydimethylsiloxane (PDMS) and poly(glycerol sebacate) (PGS) microvascular constructs with reentrant microchannels that facilitates rapid, spatially homogeneous endothelial cell seeding of a high L/D (2cm/35μm; > 550:1) aspect ratio microchannels. MEMS technology was employed for the fabrication of a monolithic, elastomeric, reentrant microvascular construct. Isotropic etching and PDMS micromolding yielded a near-cylindrical microvascular channel array. A ‘stretch - seed - seal' operation was implemented for uniform incorporation of endothelial cells along the entire microvascular area of the construct yielding endothelialized microvascular networks in less than 24h. The feasibility of this endothelialization strategy and the uniformity of cellularization were established using confocal microscope imaging. Springer Science+Business Media New York, 2015 Engineered microvascular construct nationallicence Endothelial cell seeding nationallicence MEMS nationallicence Micromolding nationallicence Naik Nisarga Department of Surgery, Harvard Medical School, Beth Israel Deaconess Medical Center, 02115, Boston, MA, USA aut Hanjaya-Putra Donny Department of Surgery, Harvard Medical School, Beth Israel Deaconess Medical Center, 02115, Boston, MA, USA aut Haller Carolyn Department of Surgery, Harvard Medical School, Beth Israel Deaconess Medical Center, 02115, Boston, MA, USA aut Allen Mark School of Electrical and Computer Engineering, Georgia Institute of Technology, 30332, Atlanta, GA, USA aut Chaikof Elliot Department of Surgery, Harvard Medical School, Beth Israel Deaconess Medical Center, 02115, Boston, MA, USA aut Biomedical Microdevices Springer US; http://www.springer-ny.com 17/4(2015-08-01), 1-7 1387-2176 17:4<1 2015 17 10544 https://doi.org/10.1007/s10544-015-9990-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10544-015-9990-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Naik Nisarga Department of Surgery, Harvard Medical School, Beth Israel Deaconess Medical Center, 02115, Boston, MA, USA aut NATIONALLICENCE 700 1- Hanjaya-Putra Donny Department of Surgery, Harvard Medical School, Beth Israel Deaconess Medical Center, 02115, Boston, MA, USA aut NATIONALLICENCE 700 1- Haller Carolyn Department of Surgery, Harvard Medical School, Beth Israel Deaconess Medical Center, 02115, Boston, MA, USA aut NATIONALLICENCE 700 1- Allen Mark School of Electrical and Computer Engineering, Georgia Institute of Technology, 30332, Atlanta, GA, USA aut NATIONALLICENCE 700 1- Chaikof Elliot Department of Surgery, Harvard Medical School, Beth Israel Deaconess Medical Center, 02115, Boston, MA, USA aut NATIONALLICENCE 773 0- Biomedical Microdevices Springer US; http://www.springer-ny.com 17/4(2015-08-01), 1-7 1387-2176 17:4<1 2015 17 10544