Fluidic system for long-term in vitro culturing and monitoring of organotypic brain slices
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| 008 | 210128e20150801xx s 000 0 eng | ||
| 024 | 7 | 0 | |a 10.1007/s10544-015-9973-6 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s10544-015-9973-6 | ||
| 245 | 0 | 0 | |a Fluidic system for long-term in vitro culturing and monitoring of organotypic brain slices |h [Elektronische Daten] |c [Tanya Bakmand, Ane Troels-Smith, Maria Dimaki, Jakob Nissen, Karsten Andersen, Luigi Sasso, Helle Waagepetersen, Jan Gramsbergen, Winnie Svendsen] |
| 520 | 3 | |a Brain slice preparations cultured in vitro have long been used as a simplified model for studying brain development, electrophysiology, neurodegeneration and neuroprotection. In this paper an open fluidic system developed for improved long term culturing of organotypic brain slices is presented. The positive effect of continuous flow of growth medium, and thus stability of the glucose concentration and waste removal, is simulated and compared to the effect of stagnant medium that is most often used in tissue culturing. Furthermore, placement of the tissue slices in the developed device was studied by numerical simulations in order to optimize the nutrient distribution. The device was tested by culturing transverse hippocampal slices from 7 days old NMRI mice for a duration of 14 days. The slices were inspected visually and the slices cultured in the fluidic system appeared to have preserved their structure better than the control slices cultured using the standard interface method. | |
| 540 | |a Springer Science+Business Media New York, 2015 | ||
| 690 | 7 | |a Tissue culturing |2 nationallicence | |
| 690 | 7 | |a Interface culturing |2 nationallicence | |
| 690 | 7 | |a Hippocampus |2 nationallicence | |
| 690 | 7 | |a Fluidic |2 nationallicence | |
| 700 | 1 | |a Bakmand |D Tanya |u Department of Micro- and Nanotechnology, Technical University of Denmark, 2800, Kgs. Lyngby, Ørsteds Plads, Denmark |4 aut | |
| 700 | 1 | |a Troels-Smith |D Ane |u Department of Micro- and Nanotechnology, Technical University of Denmark, 2800, Kgs. Lyngby, Ørsteds Plads, Denmark |4 aut | |
| 700 | 1 | |a Dimaki |D Maria |u Department of Micro- and Nanotechnology, Technical University of Denmark, 2800, Kgs. Lyngby, Ørsteds Plads, Denmark |4 aut | |
| 700 | 1 | |a Nissen |D Jakob |u Faculty of Health and Medical Sciences, University of Copenhagen, 2100, Copenhagen E, Jagtvej 160, Denmark |4 aut | |
| 700 | 1 | |a Andersen |D Karsten |u Department of Micro- and Nanotechnology, Technical University of Denmark, 2800, Kgs. Lyngby, Ørsteds Plads, Denmark |4 aut | |
| 700 | 1 | |a Sasso |D Luigi |u Department of Micro- and Nanotechnology, Technical University of Denmark, 2800, Kgs. Lyngby, Ørsteds Plads, Denmark |4 aut | |
| 700 | 1 | |a Waagepetersen |D Helle |u Faculty of Health and Medical Sciences, University of Copenhagen, 2100, Copenhagen E, Jagtvej 160, Denmark |4 aut | |
| 700 | 1 | |a Gramsbergen |D Jan |u Institute of Molecular Medicine, University of Southern Denmark, J.B. Winsløws Vej 21, 5000, Odense C, Denmark |4 aut | |
| 700 | 1 | |a Svendsen |D Winnie |u Department of Micro- and Nanotechnology, Technical University of Denmark, 2800, Kgs. Lyngby, Ørsteds Plads, Denmark |4 aut | |
| 773 | 0 | |t Biomedical Microdevices |d Springer US; http://www.springer-ny.com |g 17/4(2015-08-01), 1-7 |x 1387-2176 |q 17:4<1 |1 2015 |2 17 |o 10544 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s10544-015-9973-6 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s10544-015-9973-6 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Bakmand |D Tanya |u Department of Micro- and Nanotechnology, Technical University of Denmark, 2800, Kgs. Lyngby, Ørsteds Plads, Denmark |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Troels-Smith |D Ane |u Department of Micro- and Nanotechnology, Technical University of Denmark, 2800, Kgs. Lyngby, Ørsteds Plads, Denmark |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Dimaki |D Maria |u Department of Micro- and Nanotechnology, Technical University of Denmark, 2800, Kgs. Lyngby, Ørsteds Plads, Denmark |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Nissen |D Jakob |u Faculty of Health and Medical Sciences, University of Copenhagen, 2100, Copenhagen E, Jagtvej 160, Denmark |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Andersen |D Karsten |u Department of Micro- and Nanotechnology, Technical University of Denmark, 2800, Kgs. Lyngby, Ørsteds Plads, Denmark |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Sasso |D Luigi |u Department of Micro- and Nanotechnology, Technical University of Denmark, 2800, Kgs. Lyngby, Ørsteds Plads, Denmark |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Waagepetersen |D Helle |u Faculty of Health and Medical Sciences, University of Copenhagen, 2100, Copenhagen E, Jagtvej 160, Denmark |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Gramsbergen |D Jan |u Institute of Molecular Medicine, University of Southern Denmark, J.B. Winsløws Vej 21, 5000, Odense C, Denmark |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Svendsen |D Winnie |u Department of Micro- and Nanotechnology, Technical University of Denmark, 2800, Kgs. Lyngby, Ørsteds Plads, Denmark |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Biomedical Microdevices |d Springer US; http://www.springer-ny.com |g 17/4(2015-08-01), 1-7 |x 1387-2176 |q 17:4<1 |1 2015 |2 17 |o 10544 | ||