caa a22 4500 605479917 CHVBK 20210128100412.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s10544-015-9973-6 doi (NATIONALLICENCE)springer-10.1007/s10544-015-9973-6 Fluidic system for long-term in vitro culturing and monitoring of organotypic brain slices [Elektronische Daten] [Tanya Bakmand, Ane Troels-Smith, Maria Dimaki, Jakob Nissen, Karsten Andersen, Luigi Sasso, Helle Waagepetersen, Jan Gramsbergen, Winnie Svendsen] Brain slice preparations cultured in vitro have long been used as a simplified model for studying brain development, electrophysiology, neurodegeneration and neuroprotection. In this paper an open fluidic system developed for improved long term culturing of organotypic brain slices is presented. The positive effect of continuous flow of growth medium, and thus stability of the glucose concentration and waste removal, is simulated and compared to the effect of stagnant medium that is most often used in tissue culturing. Furthermore, placement of the tissue slices in the developed device was studied by numerical simulations in order to optimize the nutrient distribution. The device was tested by culturing transverse hippocampal slices from 7 days old NMRI mice for a duration of 14 days. The slices were inspected visually and the slices cultured in the fluidic system appeared to have preserved their structure better than the control slices cultured using the standard interface method. Springer Science+Business Media New York, 2015 Tissue culturing nationallicence Interface culturing nationallicence Hippocampus nationallicence Fluidic nationallicence Bakmand Tanya Department of Micro- and Nanotechnology, Technical University of Denmark, 2800, Kgs. Lyngby, Ørsteds Plads, Denmark aut Troels-Smith Ane Department of Micro- and Nanotechnology, Technical University of Denmark, 2800, Kgs. Lyngby, Ørsteds Plads, Denmark aut Dimaki Maria Department of Micro- and Nanotechnology, Technical University of Denmark, 2800, Kgs. Lyngby, Ørsteds Plads, Denmark aut Nissen Jakob Faculty of Health and Medical Sciences, University of Copenhagen, 2100, Copenhagen E, Jagtvej 160, Denmark aut Andersen Karsten Department of Micro- and Nanotechnology, Technical University of Denmark, 2800, Kgs. Lyngby, Ørsteds Plads, Denmark aut Sasso Luigi Department of Micro- and Nanotechnology, Technical University of Denmark, 2800, Kgs. Lyngby, Ørsteds Plads, Denmark aut Waagepetersen Helle Faculty of Health and Medical Sciences, University of Copenhagen, 2100, Copenhagen E, Jagtvej 160, Denmark aut Gramsbergen Jan Institute of Molecular Medicine, University of Southern Denmark, J.B. Winsløws Vej 21, 5000, Odense C, Denmark aut Svendsen Winnie Department of Micro- and Nanotechnology, Technical University of Denmark, 2800, Kgs. Lyngby, Ørsteds Plads, Denmark aut Biomedical Microdevices Springer US; http://www.springer-ny.com 17/4(2015-08-01), 1-7 1387-2176 17:4<1 2015 17 10544 https://doi.org/10.1007/s10544-015-9973-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10544-015-9973-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bakmand Tanya Department of Micro- and Nanotechnology, Technical University of Denmark, 2800, Kgs. Lyngby, Ørsteds Plads, Denmark aut NATIONALLICENCE 700 1- Troels-Smith Ane Department of Micro- and Nanotechnology, Technical University of Denmark, 2800, Kgs. Lyngby, Ørsteds Plads, Denmark aut NATIONALLICENCE 700 1- Dimaki Maria Department of Micro- and Nanotechnology, Technical University of Denmark, 2800, Kgs. Lyngby, Ørsteds Plads, Denmark aut NATIONALLICENCE 700 1- Nissen Jakob Faculty of Health and Medical Sciences, University of Copenhagen, 2100, Copenhagen E, Jagtvej 160, Denmark aut NATIONALLICENCE 700 1- Andersen Karsten Department of Micro- and Nanotechnology, Technical University of Denmark, 2800, Kgs. Lyngby, Ørsteds Plads, Denmark aut NATIONALLICENCE 700 1- Sasso Luigi Department of Micro- and Nanotechnology, Technical University of Denmark, 2800, Kgs. Lyngby, Ørsteds Plads, Denmark aut NATIONALLICENCE 700 1- Waagepetersen Helle Faculty of Health and Medical Sciences, University of Copenhagen, 2100, Copenhagen E, Jagtvej 160, Denmark aut NATIONALLICENCE 700 1- Gramsbergen Jan Institute of Molecular Medicine, University of Southern Denmark, J.B. Winsløws Vej 21, 5000, Odense C, Denmark aut NATIONALLICENCE 700 1- Svendsen Winnie Department of Micro- and Nanotechnology, Technical University of Denmark, 2800, Kgs. Lyngby, Ørsteds Plads, Denmark aut NATIONALLICENCE 773 0- Biomedical Microdevices Springer US; http://www.springer-ny.com 17/4(2015-08-01), 1-7 1387-2176 17:4<1 2015 17 10544