caa a22 4500 605479941 CHVBK 20210128100412.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s10544-015-9987-0 doi (NATIONALLICENCE)springer-10.1007/s10544-015-9987-0 Acoustic micromixing increases antibody-antigen binding in immunoassays [Elektronische Daten] [Yuan Gao, Phong Tran, Karolina Petkovic-Duran, Tony Swallow, Yonggang Zhu] Sound wave-assisted acoustic micromixing has been shown to increase the binding of molecules in small volumes (10-100μL) where effective mixing is difficult to achieve through conventional techniques. The aim of this work is to study whether acoustic micromixing can increase the binding efficiency of antibodies to their antigens, a reaction that forms the basis of immunoassays, including enzyme-linked immunosorbent assay (ELISA). Using a procedure from a general ELISA and immobilizing an antigen on wells of 96-well plates, it was found that acoustic micromixing at 125-150Hz increased the initial rate of antibody-antigen binding by over 80% and the total binding at the end point (i.e., 45min) by over 50%. As a result, acoustic micromixing achieved a binding level in 9min that would otherwise take 45min on a standard platform rocking mixer. Therefore acoustic micromixing has the potential to increase the detection sensitivity of ELISA as well as shorten the antigen-antibody binding times from typically 45-60min to 15min. Springer Science+Business Media New York, 2015 Acoustic nationallicence Mixing nationallicence Micromixing nationallicence Antibody-antigen interaction nationallicence ELISA nationallicence Gao Yuan Manufacturing Flagship, Commonwealth Scientific and Industrial Research Organization (CSIRO), Bayview Ave, 3168, Clayton, VIC, Australia aut Tran Phong Manufacturing Flagship, Commonwealth Scientific and Industrial Research Organization (CSIRO), Bayview Ave, 3168, Clayton, VIC, Australia aut Petkovic-Duran Karolina Manufacturing Flagship, Commonwealth Scientific and Industrial Research Organization (CSIRO), Bayview Ave, 3168, Clayton, VIC, Australia aut Swallow Tony Manufacturing Flagship, Commonwealth Scientific and Industrial Research Organization (CSIRO), Bayview Ave, 3168, Clayton, VIC, Australia aut Zhu Yonggang Manufacturing Flagship, Commonwealth Scientific and Industrial Research Organization (CSIRO), Bayview Ave, 3168, Clayton, VIC, Australia aut Biomedical Microdevices Springer US; http://www.springer-ny.com 17/4(2015-08-01), 1-5 1387-2176 17:4<1 2015 17 10544 https://doi.org/10.1007/s10544-015-9987-0 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10544-015-9987-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gao Yuan Manufacturing Flagship, Commonwealth Scientific and Industrial Research Organization (CSIRO), Bayview Ave, 3168, Clayton, VIC, Australia aut NATIONALLICENCE 700 1- Tran Phong Manufacturing Flagship, Commonwealth Scientific and Industrial Research Organization (CSIRO), Bayview Ave, 3168, Clayton, VIC, Australia aut NATIONALLICENCE 700 1- Petkovic-Duran Karolina Manufacturing Flagship, Commonwealth Scientific and Industrial Research Organization (CSIRO), Bayview Ave, 3168, Clayton, VIC, Australia aut NATIONALLICENCE 700 1- Swallow Tony Manufacturing Flagship, Commonwealth Scientific and Industrial Research Organization (CSIRO), Bayview Ave, 3168, Clayton, VIC, Australia aut NATIONALLICENCE 700 1- Zhu Yonggang Manufacturing Flagship, Commonwealth Scientific and Industrial Research Organization (CSIRO), Bayview Ave, 3168, Clayton, VIC, Australia aut NATIONALLICENCE 773 0- Biomedical Microdevices Springer US; http://www.springer-ny.com 17/4(2015-08-01), 1-5 1387-2176 17:4<1 2015 17 10544