caa a22 4500 60547995X CHVBK 20210128100412.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s10544-015-9968-3 doi (NATIONALLICENCE)springer-10.1007/s10544-015-9968-3 Testicular biodistribution of silica-gold nanoparticles after intramuscular injection in mice [Elektronische Daten] [Lara Leclerc, Jean-Philippe Klein, Valérie Forest, Delphine Boudard, Matteo Martini, Jérémie Pourchez, Marie-Geneviève Blanchin, Michèle Cottier] With the continuing development of nanomaterials, the assessment of their potential impact on human health, and especially human reproductive toxicity, is a major issue. The testicular biodistribution of nanoparticles remains poorly studied. This study investigated whether gold-silica nanoparticles could be detected in mouse testes after intramuscular injection, with a particular focus on their ability to cross the blood-testis barrier. To that purpose, well-characterized 70-nm gold core-silica shell nanoparticles were used to ensure sensitive detection using high-resolution techniques. Testes were collected at different time points corresponding to spermatogenesis stages in mice. Transmission electron microscopy and confocal microscopy were used for nanoparticle detection, and nanoparticle quantification was performed by atomic emission spectroscopy. All these techniques showed that no particles were able to reach the testes. Results accorded with the normal histological appearance of testes even at 45days post sacrifice. High-resolution techniques did not detect 70-nm silica-gold nanoparticles in mouse testes after intramuscular injection. These results are reassuring about the safety of nanoparticles with regard to male human reproduction, especially in the context of nanomedicine. Springer Science+Business Media New York, 2015 Nanoparticles nationallicence Mice nationallicence Testes nationallicence Intramuscular injection nationallicence Reproductive toxicity nationallicence Leclerc Lara University of Lyon, Jean Monnet University, EA 4624 - LINA, SFR FED 4166 - IFRESIS, 42023, Saint-Etienne, France aut Klein Jean-Philippe University of Lyon, Jean Monnet University, EA 4624 - LINA, SFR FED 4166 - IFRESIS, 42023, Saint-Etienne, France aut Forest Valérie Ecole Nationale Supérieure des Mines, CIS-EMSE, EA 4624 - LINA, SFR FED 4166 - IFRESIS, 42023, Saint-Etienne, France aut Boudard Delphine University of Lyon, Jean Monnet University, EA 4624 - LINA, SFR FED 4166 - IFRESIS, 42023, Saint-Etienne, France aut Martini Matteo Institut Lumière Matière, UMR 5306 Université Claude Bernard Lyon1 - CNRS, Université de Lyon, 69622, Villeurbanne cedex, France aut Pourchez Jérémie Ecole Nationale Supérieure des Mines, CIS-EMSE, EA 4624 - LINA, SFR FED 4166 - IFRESIS, 42023, Saint-Etienne, France aut Blanchin Marie-Geneviève Institut Lumière Matière, UMR 5306 Université Claude Bernard Lyon1 - CNRS, Université de Lyon, 69622, Villeurbanne cedex, France aut Cottier Michèle University of Lyon, Jean Monnet University, EA 4624 - LINA, SFR FED 4166 - IFRESIS, 42023, Saint-Etienne, France aut Biomedical Microdevices Springer US; http://www.springer-ny.com 17/4(2015-08-01), 1-11 1387-2176 17:4<1 2015 17 10544 https://doi.org/10.1007/s10544-015-9968-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10544-015-9968-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Leclerc Lara University of Lyon, Jean Monnet University, EA 4624 - LINA, SFR FED 4166 - IFRESIS, 42023, Saint-Etienne, France aut NATIONALLICENCE 700 1- Klein Jean-Philippe University of Lyon, Jean Monnet University, EA 4624 - LINA, SFR FED 4166 - IFRESIS, 42023, Saint-Etienne, France aut NATIONALLICENCE 700 1- Forest Valérie Ecole Nationale Supérieure des Mines, CIS-EMSE, EA 4624 - LINA, SFR FED 4166 - IFRESIS, 42023, Saint-Etienne, France aut NATIONALLICENCE 700 1- Boudard Delphine University of Lyon, Jean Monnet University, EA 4624 - LINA, SFR FED 4166 - IFRESIS, 42023, Saint-Etienne, France aut NATIONALLICENCE 700 1- Martini Matteo Institut Lumière Matière, UMR 5306 Université Claude Bernard Lyon1 - CNRS, Université de Lyon, 69622, Villeurbanne cedex, France aut NATIONALLICENCE 700 1- Pourchez Jérémie Ecole Nationale Supérieure des Mines, CIS-EMSE, EA 4624 - LINA, SFR FED 4166 - IFRESIS, 42023, Saint-Etienne, France aut NATIONALLICENCE 700 1- Blanchin Marie-Geneviève Institut Lumière Matière, UMR 5306 Université Claude Bernard Lyon1 - CNRS, Université de Lyon, 69622, Villeurbanne cedex, France aut NATIONALLICENCE 700 1- Cottier Michèle University of Lyon, Jean Monnet University, EA 4624 - LINA, SFR FED 4166 - IFRESIS, 42023, Saint-Etienne, France aut NATIONALLICENCE 773 0- Biomedical Microdevices Springer US; http://www.springer-ny.com 17/4(2015-08-01), 1-11 1387-2176 17:4<1 2015 17 10544