Microelectrode bioimpedance analysis distinguishes basal and claudin-low subtypes of triple negative breast cancer cells
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| 024 | 7 | 0 | |a 10.1007/s10544-015-9977-2 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s10544-015-9977-2 | ||
| 245 | 0 | 0 | |a Microelectrode bioimpedance analysis distinguishes basal and claudin-low subtypes of triple negative breast cancer cells |h [Elektronische Daten] |c [Vaishnavi Srinivasaraghavan, Jeannine Strobl, Masoud Agah] |
| 520 | 3 | |a Triple negative breast cancer (TNBC) is highly aggressive and has a poor prognosis when compared to other molecular subtypes. In particular, the claudin-low subtype of TNBC exhibits tumor-initiating/cancer stem cell like properties. Here, we seek to find new biomarkers to discriminate different forms of TNBC by characterizing their bioimpedance. A customized bioimpedance sensor with four identical branched microelectrodes with branch widths adjusted to accommodate spreading of individual cells was fabricated on silicon and pyrex/glass substrates. Cell analyses were performed on the silicon devices which showed somewhat improved inter-electrode and intra-device reliability. We performed detailed analysis of the bioimpedance spectra of four TNBC cell lines, comparing the peak magnitude, peak frequency and peak phase angle between claudin-low TNBC subtype represented by MDA-MB-231 and Hs578T with that of two basal cells types, the TNBC MDA-MB-468, and an immortalized non-malignant basal breast cell line, MCF-10A. The claudin-low TNBC cell lines showed significantly higher peak frequencies and peak phase angles than the properties might be useful in distinguishing the clinically significant claudin-low subtype of TNBC. | |
| 540 | |a Springer Science+Business Media New York, 2015 | ||
| 690 | 7 | |a Bioimpedance |2 nationallicence | |
| 690 | 7 | |a Breast cancer |2 nationallicence | |
| 690 | 7 | |a ECIS |2 nationallicence | |
| 690 | 7 | |a Cell-cell junctions |2 nationallicence | |
| 690 | 7 | |a TNBC |2 nationallicence | |
| 690 | 7 | |a Claudin-low |2 nationallicence | |
| 700 | 1 | |a Srinivasaraghavan |D Vaishnavi |u The Bradley Department of Electrical and Computer Engineering, Virginia Tech, 302, Whittemore Hall, 24061, Blacksburg, VA, USA |4 aut | |
| 700 | 1 | |a Strobl |D Jeannine |u The Bradley Department of Electrical and Computer Engineering, Virginia Tech, 302, Whittemore Hall, 24061, Blacksburg, VA, USA |4 aut | |
| 700 | 1 | |a Agah |D Masoud |u The Bradley Department of Electrical and Computer Engineering, Virginia Tech, 302, Whittemore Hall, 24061, Blacksburg, VA, USA |4 aut | |
| 773 | 0 | |t Biomedical Microdevices |d Springer US; http://www.springer-ny.com |g 17/4(2015-08-01), 1-11 |x 1387-2176 |q 17:4<1 |1 2015 |2 17 |o 10544 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s10544-015-9977-2 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s10544-015-9977-2 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Srinivasaraghavan |D Vaishnavi |u The Bradley Department of Electrical and Computer Engineering, Virginia Tech, 302, Whittemore Hall, 24061, Blacksburg, VA, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Strobl |D Jeannine |u The Bradley Department of Electrical and Computer Engineering, Virginia Tech, 302, Whittemore Hall, 24061, Blacksburg, VA, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Agah |D Masoud |u The Bradley Department of Electrical and Computer Engineering, Virginia Tech, 302, Whittemore Hall, 24061, Blacksburg, VA, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Biomedical Microdevices |d Springer US; http://www.springer-ny.com |g 17/4(2015-08-01), 1-11 |x 1387-2176 |q 17:4<1 |1 2015 |2 17 |o 10544 | ||