caa a22 4500 60548001X CHVBK 20210128100413.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s10544-015-9977-2 doi (NATIONALLICENCE)springer-10.1007/s10544-015-9977-2 Microelectrode bioimpedance analysis distinguishes basal and claudin-low subtypes of triple negative breast cancer cells [Elektronische Daten] [Vaishnavi Srinivasaraghavan, Jeannine Strobl, Masoud Agah] Triple negative breast cancer (TNBC) is highly aggressive and has a poor prognosis when compared to other molecular subtypes. In particular, the claudin-low subtype of TNBC exhibits tumor-initiating/cancer stem cell like properties. Here, we seek to find new biomarkers to discriminate different forms of TNBC by characterizing their bioimpedance. A customized bioimpedance sensor with four identical branched microelectrodes with branch widths adjusted to accommodate spreading of individual cells was fabricated on silicon and pyrex/glass substrates. Cell analyses were performed on the silicon devices which showed somewhat improved inter-electrode and intra-device reliability. We performed detailed analysis of the bioimpedance spectra of four TNBC cell lines, comparing the peak magnitude, peak frequency and peak phase angle between claudin-low TNBC subtype represented by MDA-MB-231 and Hs578T with that of two basal cells types, the TNBC MDA-MB-468, and an immortalized non-malignant basal breast cell line, MCF-10A. The claudin-low TNBC cell lines showed significantly higher peak frequencies and peak phase angles than the properties might be useful in distinguishing the clinically significant claudin-low subtype of TNBC. Springer Science+Business Media New York, 2015 Bioimpedance nationallicence Breast cancer nationallicence ECIS nationallicence Cell-cell junctions nationallicence TNBC nationallicence Claudin-low nationallicence Srinivasaraghavan Vaishnavi The Bradley Department of Electrical and Computer Engineering, Virginia Tech, 302, Whittemore Hall, 24061, Blacksburg, VA, USA aut Strobl Jeannine The Bradley Department of Electrical and Computer Engineering, Virginia Tech, 302, Whittemore Hall, 24061, Blacksburg, VA, USA aut Agah Masoud The Bradley Department of Electrical and Computer Engineering, Virginia Tech, 302, Whittemore Hall, 24061, Blacksburg, VA, USA aut Biomedical Microdevices Springer US; http://www.springer-ny.com 17/4(2015-08-01), 1-11 1387-2176 17:4<1 2015 17 10544 https://doi.org/10.1007/s10544-015-9977-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10544-015-9977-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Srinivasaraghavan Vaishnavi The Bradley Department of Electrical and Computer Engineering, Virginia Tech, 302, Whittemore Hall, 24061, Blacksburg, VA, USA aut NATIONALLICENCE 700 1- Strobl Jeannine The Bradley Department of Electrical and Computer Engineering, Virginia Tech, 302, Whittemore Hall, 24061, Blacksburg, VA, USA aut NATIONALLICENCE 700 1- Agah Masoud The Bradley Department of Electrical and Computer Engineering, Virginia Tech, 302, Whittemore Hall, 24061, Blacksburg, VA, USA aut NATIONALLICENCE 773 0- Biomedical Microdevices Springer US; http://www.springer-ny.com 17/4(2015-08-01), 1-11 1387-2176 17:4<1 2015 17 10544