Integrin associated proteins differentially regulate neutrophil polarity and directed migration in 2D and 3D
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| 024 | 7 | 0 | |a 10.1007/s10544-015-9998-x |2 doi |
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| 245 | 0 | 0 | |a Integrin associated proteins differentially regulate neutrophil polarity and directed migration in 2D and 3D |h [Elektronische Daten] |c [Yukie Yamahashi, Peter Cavnar, Laurel Hind, Erwin Berthier, David Bennin, David Beebe, Anna Huttenlocher] |
| 520 | 3 | |a Directed neutrophil migration in blood vessels and tissues is critical for proper immune function; however, the mechanisms that regulate three-dimensional neutrophil chemotaxis remain unclear. It has been shown that integrins are dispensable for interstitial three-dimensional (3D) leukocyte migration; however, the role of integrin regulatory proteins during directed neutrophil migration is not known. Using a novel microfluidic gradient generator amenable to 2D and 3D analysis, we found that the integrin regulatory proteins Kindlin-3, RIAM, and talin-1 differentially regulate neutrophil polarization and directed migration to gradients of chemoattractant in 2D versus 3D. Both talin-1-deficient and RIAM-deficient neutrophil-like cells had impaired adhesion, polarization, and migration on 2D surfaces whereas in 3D the cells polarized but had impaired 3D chemotactic velocity. Kindlin-3 deficient cells were able to polarize and migrate on 2D surfaces but had impaired directionality. In a 3D environment, Kindlin-3 deficient cells displayed efficient chemotaxis. These findings demonstrate that the role of integrin regulatory proteins in cell polarity and directed migration can be different in 2D and 3D. | |
| 540 | |a Springer Science+Business Media New York, 2015 | ||
| 690 | 7 | |a Neutrophil |2 nationallicence | |
| 690 | 7 | |a Chemotaxis |2 nationallicence | |
| 690 | 7 | |a Integrin regulatory proteins |2 nationallicence | |
| 690 | 7 | |a Microfluidics |2 nationallicence | |
| 700 | 1 | |a Yamahashi |D Yukie |u Department of Pediatrics, University of Wisconsin-Madison, Madison, WI, USA |4 aut | |
| 700 | 1 | |a Cavnar |D Peter |u Department of Biology, University of West Florida, Pensacola, FL, USA |4 aut | |
| 700 | 1 | |a Hind |D Laurel |u Department of Medical Microbiology and Immunology, University of Wisconsin-Madison, 1550 Linden Drive, Room 4205 MSB, 53706, Madison, WI, USA |4 aut | |
| 700 | 1 | |a Berthier |D Erwin |u Department of Bioengineering, University of Wisconsin-Madison, Madison, WI, USA |4 aut | |
| 700 | 1 | |a Bennin |D David |u Department of Pediatrics, University of Wisconsin-Madison, Madison, WI, USA |4 aut | |
| 700 | 1 | |a Beebe |D David |u Department of Bioengineering, University of Wisconsin-Madison, Madison, WI, USA |4 aut | |
| 700 | 1 | |a Huttenlocher |D Anna |u Department of Pediatrics, University of Wisconsin-Madison, Madison, WI, USA |4 aut | |
| 773 | 0 | |t Biomedical Microdevices |d Springer US; http://www.springer-ny.com |g 17/5(2015-10-01), 1-9 |x 1387-2176 |q 17:5<1 |1 2015 |2 17 |o 10544 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s10544-015-9998-x |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s10544-015-9998-x |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Yamahashi |D Yukie |u Department of Pediatrics, University of Wisconsin-Madison, Madison, WI, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Cavnar |D Peter |u Department of Biology, University of West Florida, Pensacola, FL, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Hind |D Laurel |u Department of Medical Microbiology and Immunology, University of Wisconsin-Madison, 1550 Linden Drive, Room 4205 MSB, 53706, Madison, WI, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Berthier |D Erwin |u Department of Bioengineering, University of Wisconsin-Madison, Madison, WI, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Bennin |D David |u Department of Pediatrics, University of Wisconsin-Madison, Madison, WI, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Beebe |D David |u Department of Bioengineering, University of Wisconsin-Madison, Madison, WI, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Huttenlocher |D Anna |u Department of Pediatrics, University of Wisconsin-Madison, Madison, WI, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Biomedical Microdevices |d Springer US; http://www.springer-ny.com |g 17/5(2015-10-01), 1-9 |x 1387-2176 |q 17:5<1 |1 2015 |2 17 |o 10544 | ||