caa a22 4500 605480079 CHVBK 20210128100413.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s10544-015-9993-2 doi (NATIONALLICENCE)springer-10.1007/s10544-015-9993-2 A multilayered microfluidic blood vessel-like structure [Elektronische Daten] [Anwarul Hasan, Arghya Paul, Adnan Memic, Ali Khademhosseini] There is an immense need for tissue engineered blood vessels. However, current tissue engineering approaches still lack the ability to build native blood vessel-like perfusable structures with multi-layered vascular walls. This paper demonstrated a new method to fabricate tri-layer biomimetic blood vessel-like structures on a microfluidic platform using photocrosslinkable gelatin hydrogel. The presented method enables fabrication of physiological blood vessel-like structures with mono-, bi- or tri-layer vascular walls. The diameter of the vessels, the total thickness of the vessel wall and the thickness of each individual layer of the wall were independently controlled. The developed fabrication process is a simple and rapid method, allowing the physical fabrication of the vascular structure in minutes, and the formation of a vascular endothelial cell layer inside the vessels in 3-5days. The fabricated vascular constructs can potentially be used in numerous applications including drug screening, development of in vitro models for cardiovascular diseases and/or cancer metastasis, and study of vascular biology and mechanobiology. Springer Science+Business Media New York, 2015 Tissue engineering nationallicence Microfluidics nationallicence Blood vessel nationallicence Hydrogel nationallicence PDMS nationallicence Microfabrication nationallicence Hasan Anwarul Biomedical Engineering, and Department of Mechanical Engineering, Faculty of Engineering and Architecture, American University of Beirut, 1107 2020, Beirut, Lebanon aut Paul Arghya Biomaterials Innovation Research Center, Division of Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, 02139, Cambridge, MA, USA aut Memic Adnan Center of Nanotechnology, King Abdulaziz University, 21589, Jeddah, Saudi Arabia aut Khademhosseini Ali Biomaterials Innovation Research Center, Division of Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, 02139, Cambridge, MA, USA aut Biomedical Microdevices Springer US; http://www.springer-ny.com 17/5(2015-10-01), 1-13 1387-2176 17:5<1 2015 17 10544 https://doi.org/10.1007/s10544-015-9993-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10544-015-9993-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hasan Anwarul Biomedical Engineering, and Department of Mechanical Engineering, Faculty of Engineering and Architecture, American University of Beirut, 1107 2020, Beirut, Lebanon aut NATIONALLICENCE 700 1- Paul Arghya Biomaterials Innovation Research Center, Division of Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, 02139, Cambridge, MA, USA aut NATIONALLICENCE 700 1- Memic Adnan Center of Nanotechnology, King Abdulaziz University, 21589, Jeddah, Saudi Arabia aut NATIONALLICENCE 700 1- Khademhosseini Ali Biomaterials Innovation Research Center, Division of Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, 02139, Cambridge, MA, USA aut NATIONALLICENCE 773 0- Biomedical Microdevices Springer US; http://www.springer-ny.com 17/5(2015-10-01), 1-13 1387-2176 17:5<1 2015 17 10544