caa a22 4500 605480109 CHVBK 20210128100413.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s10544-015-0005-3 doi (NATIONALLICENCE)springer-10.1007/s10544-015-0005-3 Influence of surface modification and static pressure on microdialysis protein extraction efficiency [Elektronische Daten] [Jiangtao Chu, Torgny Undin, Sara Lind, Klas Hjort, Andreas Dahlin] There is growing interest in using microdialysis (MD) for monitoring larger and more complex molecules such as neuropeptides and proteins. This promotes the use of MD membranes with molecular weight cut off (MWCO) of 100kDa or above. The hydrodynamic property of the membrane goes to ultrafiltration or beyond, making the MD catheters more sensitive to pressure. In the meantime, despite the large pore size, studies have shown that membrane biofouling still lead to unstable catheter performance. The objective is to study in vitro how 500kDa dextran and Poloxamer 407 surface modification affect the fluid recovery (FR) and extraction efficiency (EE) of 100kDa MWCO MD catheters. A pressure chamber was designed to facilitate the tests, using as MD sample a protein standard with similar concentrations as in human cerebral spinal fluid, comparing native and Poloxamer 407 modified MD catheters. The collected dialysate fractions were examined for FR and protein EE, employing Dot-it Spot-it Protein Assay for total protein EE and targeted mass spectrometry (MS) for EE of individual proteins and peptides. The FR results suggested that the surface modified catheters were less sensitive to the pressure and provide higher precision, and provided a FR closer to 100%. The surface modification did not show a significant effect on the protein EE. The average total protein EE of surface modified catheters was slightly higher than that of the native ones. The MS EE data of individual proteins showed a clear trend of complex response in EE with pressure. Springer Science+Business Media New York, 2015 Microdialysis nationallicence Surface modification nationallicence Poloxamer nationallicence Protein nationallicence Extraction efficiency nationallicence Chu Jiangtao Department of Engineering Sciences, Uppsala University, Box 534, SE-751 21, Uppsala, Sweden aut Undin Torgny Department of Chemistry-BMC, Analytical Chemistry and Science for Life Laboratory, Uppsala University, Box 599, SE-751 24, Uppsala, Sweden aut Lind Sara Department of Chemistry-BMC, Analytical Chemistry and Science for Life Laboratory, Uppsala University, Box 599, SE-751 24, Uppsala, Sweden aut Hjort Klas Department of Engineering Sciences, Uppsala University, Box 534, SE-751 21, Uppsala, Sweden aut Dahlin Andreas Department of Engineering Sciences, Uppsala University, Box 534, SE-751 21, Uppsala, Sweden aut Biomedical Microdevices Springer US; http://www.springer-ny.com 17/5(2015-10-01), 1-11 1387-2176 17:5<1 2015 17 10544 https://doi.org/10.1007/s10544-015-0005-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10544-015-0005-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chu Jiangtao Department of Engineering Sciences, Uppsala University, Box 534, SE-751 21, Uppsala, Sweden aut NATIONALLICENCE 700 1- Undin Torgny Department of Chemistry-BMC, Analytical Chemistry and Science for Life Laboratory, Uppsala University, Box 599, SE-751 24, Uppsala, Sweden aut NATIONALLICENCE 700 1- Lind Sara Department of Chemistry-BMC, Analytical Chemistry and Science for Life Laboratory, Uppsala University, Box 599, SE-751 24, Uppsala, Sweden aut NATIONALLICENCE 700 1- Hjort Klas Department of Engineering Sciences, Uppsala University, Box 534, SE-751 21, Uppsala, Sweden aut NATIONALLICENCE 700 1- Dahlin Andreas Department of Engineering Sciences, Uppsala University, Box 534, SE-751 21, Uppsala, Sweden aut NATIONALLICENCE 773 0- Biomedical Microdevices Springer US; http://www.springer-ny.com 17/5(2015-10-01), 1-11 1387-2176 17:5<1 2015 17 10544